摘要
目的研究白色念珠菌、茄病镰刀菌、烟曲霉菌在角膜内的生长方式。方法应用表层角膜植片术,在植床与植片间注射真菌菌液,建立浅层真菌性角膜感染的兔模型。28只健康成年家兔,分为白色念珠菌感染组、茄病镰刀菌感染组、烟曲霉菌感染组,每组随机取8只兔,并设立4只兔为对照组。进行组织病理学及透射电镜观察。结果大体观察发现3种真菌感染的临床表现各有特点。组织病理学及透射电镜检测可见白色念珠菌垂直生长,茄病镰刀菌平行于角膜板层生长,烟曲霉菌斜行生长。结论3种不同真菌在角膜内生长方式不同,可能存在不同的致病机制。
Objective The prevalence of fungal keratitis have increased gradually in recent years. Many kinds of fungi have many different symptoms, which make fungal keratitls become difficulty to manage for ophthalmologist. This study was to investigate the growth statue of three major fungi in cornea. Methods The model of surficial corneal fungal infection was established by injection of fungi solution （ 0. 1 mL） into subgraft of cornea in twenty-four receipt rabbits （48 eyes） with epikeratophakia. The animal models were divided into Albicans candidis inoculation group, Fusarium solani inoculation group and Aspergillus fumigatus inoculation group,and 0. 1 mL physiology salt solution was injected in the same way in 4 control rabbits. The corneas specimen were obtained in 1 day, 3,5,8 days after inoculation for the pathological observation. Results Different clinical features were found in Albicans candidls inoculation group, Fusarium solani inoculation group and Aspergillus fumigatus inoculation group in the 1 st day following the inoculation. More new blood vessels were seen in cornea in Albicans candidis inoculation group,Fusarium solani inoculation group and Aspergillus fumigatus inoculation group on the 3rd day in comparison with control rabbits. Three kinds of growth features of fungi were found in different inoculation groups, showing the vertical-like growth,parallel-like growth and oblique-like growth fashion respectively. The filtration of white cells could been seen in different location of cornea in various groups. Conclusion Three kinds of fungi have different growth features, indicating different pathological mechanisms among different fungal keratitis.
出处
《眼科研究》
CSCD
北大核心
2008年第1期26-29,共4页
Chinese Ophthalmic Research
基金
国家自然科学基金(39470740\30271394)
山东省科技攻关计划项目(2004GG2202154)资助
