摘要
目的:研究香加皮提取单体化合物杠柳苷元对大鼠和小鼠肥大细胞脱颗粒及组胺释放的影响。方法:大鼠腹腔注射百日咳疫苗、后腿注射卵白蛋白致敏,用于测定肥大细胞脱颗粒反应及制备抗血清;取致敏大鼠的血清稀释后对小鼠进行腹腔注射,测定肥大细胞脱颗粒反应;以荧光分光光度法测定组胺浓度。结果:杠柳苷元对体外培养肥大细胞的组胺释放有显著的抑制作用,实验剂量即可使组胺释放浓度降低(69.4±8.6)%,其抑制作用呈显著的剂量依赖关系;杠柳苷元对抗原致敏大鼠肥大细胞的组胺释放也有显著的抑制作用,在20μg·mL-1浓度时即可使组胺释放浓度减少73.55%;杠柳苷元口服给予致敏小鼠后,在50mg·kg-1剂量时即可使小鼠组胺释放浓度减少80%以上,并呈显著的剂量依赖关系。结论:杠柳苷元对体外培养的肥大细胞、致敏大鼠肥大细胞的组胺释放有显著的抑制作用;口服给予杠柳苷元可使小鼠显著减少肥大细胞的组胺释放。鉴于肥大细胞脱颗粒及组胺释放在炎症反应中的作用,可认为杠柳苷元是香加皮具有抗炎作用的有效成分之一。
OBJECTIVE： To investigate the effects of Periplogenin, a compound extracted and purified from traditional Chinese medicine Cortex Periplocae, on mast cell degranulation and histamine release in mice and rats. METHODS： Sensitization was induced by injecting pertussis vaccine injected in rats＇ abdominal cavity and ovalbumin injected in mice＇s hind leg to detect mast cell degranulation reaction and prepare antiserum; rats were injected intraperitoneally with the diluted serum sample taken form sensitized rats to detect mast cell degranulation reaction; and the concentration of histamine was determined by fluorescence spectrophotometry. RESULTS： Periplogenin showed significant inhibitory effect on histamine release of mast cell cultured in vitro, and at experimental dose it could decrease histamine release by（69.4± 8.6）% in an obvious dose-dependent manner. Periplogenin also showed obvious inhibitory effect on histamine release of mast cells in antigen- sensitized rats, and at a concentration of 20 μg . mL^-1,it could decrease histamine release by 73.55%, and at an oral dose of 50 mg . kg^-1, it could dose-dependently reduce histamine release by above 80% in sensitized rats. CONCLUSION： Periplogenin showed significant inhibitory effects on histamine release of mast cells either cultured in vitro or in antigen- sensitized rats. Oral administration of Periplogenin can result in the significant reduction of histamine release of mast cells in rats. In view of the role that the mast cell histamine release and degranulation play in inflammation reaction, periplogenin can be regarded as one of the active anti-inflammation components of traditional Chinese medicine Cortex Periplocae.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第3期166-168,共3页
China Pharmacy
关键词
杠柳苷元
肥大细胞脱颗粒
组胺释放
炎症
实验研究
Periplogenin
Mast cell degranulation
Histamine release
Inflammation
Experimental study
