摘要
目的：建立以气相色谱-质谱法测定火麻壳和火麻仁中△^9-四氢大麻酚含量的方法。方法：采用HP-5石英毛细管柱，程序升温：200℃（保持1min）270℃（保持10min），升温速率为3℃·min^-1，进样口温度为280℃，检测器温度为290℃。结果：△^9-四氢大麻酚检测浓度在5．0～45．0μg·mL^-1范围内与峰面积比值呈良好的线性关系（r=0．9987）；最低检测限为1．0μg·mL^-1（S／N=5）；平均加样回收率为81．1％，RSD=3．8％（n=9）。结论：本方法简便、准确、重现性好，可用于火麻制品的质量控制。
OBJECTIVE： To determine the content of △^9- tetrahydrocannabinol （THC） in hemp by GC-MS. METH ODS： HP- 5 quartz capillary column was used. The temperature programming was as follows： the initial temperature was kept at 200 ℃ for 1 min, then raised to 270 ℃ at the rate of 3 ℃. min ^-1 and kept for 10 min; the injector temperature was 280 ℃ and the detector temperature was 290 ℃. RESULTS： The good linearity between peak area and concentration was obtained over the range of 5.0 -45.0 μg . mL^-1 （ r = 0.998 7）. The lowest detectable limit was 1.0μg . mL^-1（S/N = 5） .The average recovery rate was 81.1% （RSD = 3.8%, n = 9） . CONCLUSION： The procedure is simple, accurate and reproducible, and suitable for the quality control of hemp preparation.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第3期201-202,共2页
China Pharmacy
