摘要
目的:研究缺血后处理对缺血再灌注心肌细胞凋亡及Bcl-2和Bax蛋白表达的影响。方法:24只大耳白兔随机分成4组:假手术组(S)、缺血再灌注组(IR)、缺血预适应组(IP)和缺血后处理组(IPO)。S组(6只):开胸只穿线不结扎血管,麻醉维持120min;IR组(6只):结扎30min,再灌注120min;IP组(6只):结扎5min,再灌注5min,反复3次诱导缺血预适应,之后再按IR组操作;IPO组(6只):结扎30min后,再灌注10s、缺血10s,反复6次,再灌注120min。应用TUNEL法观察各组心肌细胞凋亡,应用免疫组化SP法观察Bcl-2和Bax蛋白的表达。结果:与IR组相比,IP组和IPO组心肌梗死范围减小,心肌细胞的凋亡指数降低(P<0.05),Bcl-2/Bax比例升高(P<0.05),而IP组和IPO组间差异无统计学意义(P>0.05)。结论:缺血后处理可以减轻再灌注心肌细胞的凋亡,这一过程可能是通过升高Bcl-2/Bax比值来实现的。
Aim： To elucidate the effects of ischemie posteonditioning on myocardial cell apoptosis and expressions of Bcl-2 and Bax protein after ischemia and reperfusion in rabbits. Methods：Rabbits were randomized into 4 groups： sham operation group（ Group S） ：only braid,with no occlusion;ischemia and reperfusion group（ Group IR） ：left anterior descending （LAD） occlusion and reperfusion,with no other intervention; myocardial ischemic preconditioning（ Group IP） ：3 cycles of myocardial isehemia （5 min） and reperfusion（5 min） preceded the ischemia and reperfusion protocol ; myocardial ischemic postconditioning（ Group IPO） ： 6 postconditioning cycles of 10 s reperfusion and 10 s ischemia after the coronary occlusion （30 min） ,then reperfusion for 120 min. Cardiomyocyte apoptosis was determined by TUNEL,and the expressions of Bcl-2 anti Bax protein in the myocardium of ischemic area were measured by immunohistochemical technique. Results： Compared with those in the Group IR, cardiomyocyte apoptosis index was significantly reduced and the ratio of Bcl-2 to Bax were increased in both Group IP anti Group IPO. No significant difference was found between Group IP and Group IPO（ P 〉 0.05 ）. Conclusion： Ischemic postconditioning,like ischcmic preconditioning, exerts an anti-apoptotic effect,which may associate with regulating the expressions of Bcl-2 and Bax protein.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2008年第1期57-59,共3页
Journal of Zhengzhou University(Medical Sciences)
基金
河南省科技攻关基金资助项目0624410084
