摘要
目的:检测人肺癌组织中Atrogin-1基因mRNA表达水平,了解对肺癌患者术后生存情况及其影响因素。方法:运用实时定量PCR技术测定60例肺癌患者的癌组织及配对的癌旁组织中Atrogin-1基因mRNA表达水平,采用Kaplan-Meier单因素与Cox多元回归进行生存分析。结果:肺癌组织中Atrogin-1 mRNA表达水平(-8.15±3.94)显著高于其配对的癌旁组织(-10.39±4.35)(t=3.873,P=0.000),其中71.67%(43/60)患者Atrogin-1 mR-NA高表达,28.33%(17/60)患者低表达。Kaplan-Meier法生存分析结果显示:Atrogin-1的表达水平、淋巴结转移、家族史、病理分级与患者的生存率显著相关。Cox回归分析结果显示:Atrogin-1 mRNA表达水平、淋巴结转移、病理分级和吸烟是影响肺癌患者生存率的独立危险因素。结论:肺癌组织中Atrogin-1的高表达可能影响肺癌患者术后的生存,其可作为肺癌患者预后的一个评价指标,并可能成为基因治疗的一个作用靶点。
Aim： To investigate Atrogin-1 mRNA expression levels in lung cancer and analyze the influencing factors of the survival of patients after operation. Methods ： We quantified Atrogin-1 mRNA expression levels in sixty pairs of lung cancer tissue and their matched normal lung tissues by using real-time PCR assay. Results： Paired-samples t-test indicated that expression of Atrogin-1 mRNA level in cancers （ -8.15 ±3.94） was significantly higher than that in matched normal ones （ -10.39±4.35）（t=3.873, P =0.000）. up-regulationofAtrogin-1 mRNA were found in 43 of 60 （71.67%） of lung cancer tissues compared with the matched normal lung tissues, down-regulation of Atrogin-1 mRNA were found in 17 of 60 （28.33%）. The Univariate survival analysis by Kaplan-Meier showed that the mRNA expression of Atrogin-1 , metastasis, smoke, family history, tumor stage, had significant association with survival rate. Cox regression survival analysis showed that the mRNA expression of Atrogin-1, metastasis, smoke, tumor stage were significantly independent factors with survival rate. Conclusion ： Up-regulation of Atrogin-1 in lung cancer may influence patient＇s prognosis, however it may be identified as a novel prognostic indicator and a potential therapeutic target.
出处
《郑州大学学报（医学版）》
CAS
北大核心
2008年第1期130-133,共4页
Journal of Zhengzhou University(Medical Sciences)
