摘要
目的采用HPLC法测定消斑颗粒中补骨脂素、异补骨脂素含量。方法色谱柱为DikmaODS-C18色谱柱,乙腈-0.025 mol/L磷酸水溶液(30∶70)为流动相,检测波长为246 nm。结果供试溶液中补骨脂素、异补骨脂素与相邻组分分离度良好,阴性液无干扰。方法重复性、稳定性、精密度、回收率试验均符合有关规定。结论方法简单、快捷、精确,可作为消斑颗粒质量控制方法之一。
Aim： To establish a HPLC method for determination of psoralen and isopsoralen in Xiaoban granule. Methods： Dikma ODS- C18 column was used, with 0. 025 mol/L H3PO4 solution/ethensol （70： 30） as a mobile phase. Absorbance detection wave length was at 246 nm. Results： The psoralen and isopsoralen in Xiaoban granule were baseline separated and determined. Conclusion： The method was simple, convenient, accurate and can be used for the quality control of Xiaoban granule.
出处
《辽宁化工》
CAS
2008年第1期62-64,共3页
Liaoning Chemical Industry
