摘要
目的探讨榴莲壳醇提物对拘束负荷诱发小鼠应激性肝损伤的保护作用。方法拘束负荷18h诱发小鼠应激性肝损伤,分别用赖氏法测定小鼠血浆丙氨酸转氨酶(ALT)活性、硫代巴比妥酸法测定血浆丙二醛(MDA)水平、HPLC法测定肝组织谷胱甘肽(GSH)含量、Griess化学法测定肝组织一氧化氮(NO)含量,荧光酶标仪测定体外抗氧化能力指数(ORAC)。结果与拘束负荷模型组相比,榴莲壳醇提物可以明显降低拘束负荷小鼠血浆ALT活性,有效地降低拘束负荷小鼠血浆MDA水平与肝组织NO含量,对肝组织GSH含量也有一定程度的改善作用;榴莲壳醇提物显示出较强的体外抗氧化能力。结论榴莲壳醇提物对拘束负荷诱发小鼠应激性肝损伤具有一定的保护作用,其作用机制可能与清除自由基和减少拘束负荷小鼠氧化应激水平有关。
Objective To investigate the protective effect of Durian shell alcohol - extracts （DSE） on mice liver injury loaded with restraint stress. Methods After 18 - h restraint stress, the alanine aminotransferase （ALT） activity in plasma was determined by Reitamn - Frankel assay. Thio - barbituric acid merthod （TBARS） was performed to detect the level of malondialdehyde （MDA） in plasma. The content of glutathione （GSH） in liver tissue was determined by HPLC method, and nitric oxide （NO） content in liver was determined by Griess method. Antioxidative capacity of DSE in vitro was measured by oxygen radical absorbanee capacity （ORAC） assay. Results Compared with the model group, DSE could markedly reduce plasma ALT activity, reduce the plasma MDA level and liver NO content, and increase hepatic GSH content. Moreover, DSE could effectively increase the anti -oxidative capacity in vitro. Conclusion Oral use of DSE exerts a protective effect on mice liver injury loaded with restraint stress , and its mechanism may be related to the clearance of free radical scavenging activity and improvement of oxidative status.
出处
《中药新药与临床药理》
CAS
CSCD
2008年第1期22-25,共4页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
国家科技支撑计划(2006BAI06A00)
关键词
榴莲壳醇提物
拘束负荷
肝损伤
保护作用
Durio zibethinus Mutt
Restraint stress
Liver injury
Protection
