摘要
目的:探讨60Coγ射线照射对肺泡II型细胞和肺泡隔间质细胞的生物效应。方法:原代分离肺内II型上皮细胞(AT-Ⅱ)和间质细胞包括巨噬细胞和成纤维细胞,分别进行0、3、5、7Gy的γ射线照射,用细胞核嗜银染色观察照射对AT-Ⅱ增殖的影响;用酶谱分析检测照射后AT-Ⅱ和间质细胞培养上清中基质金属蛋白酶-2、-9(MMP-2,-9)的活性;用ELISA检测间质细胞培养上清中TGF-β1和IV型胶原含量。结果:AT-Ⅱ的核仁数量随照射剂量增加而增多,其中7Gy组最高;AT-II培养上清中MMP-2、-9活性随照射剂量增加呈先增高后降低趋势,间质细胞上清中MMP-2、-9活性和TGF-β1水平逐渐升高,IV型胶原分泌水平呈先降低后升高趋势。结论:放射性肺损伤早期,AT-Ⅱ、巨噬细胞和成纤维细胞均参与肺组织无效性重建过程,与晚期肺纤维化启动有一定的内在联系。
AIM： To explore the biological effect of ^60Coγ ray on alveolar type Ⅱ cells and interstitial cells of alveoliar septum. METHODS： Alveolar type Ⅱ cells（AT-Ⅱ） and interstitial cells including interstitial macrophages and fibroblasts were irradiated by 0, 3, 5, 7 Gy of γ ray respectively. The effect of irradiation on AT-Ⅱ proliferation was observed by argentation against nucleus. The activity of MMP-2, -9 in supematants from AT-Ⅱ and interstitial cells after irradiation was determined by zymography. The levels of TGF-β1 and collagen type Ⅳ in supematant from interstitial cells after irradiation were measured by ELISA. RESULTS： The nucleolus number of AT-Ⅱ was increased with the increase of irradiation dose and group 7 Gy reached the highest level. The activity of MMP-2, -9 in supernatant from AT-Ⅱ after irradiation increased at first and then decreased gradually. The activity of MMP-2, -9 and the content of TGF-β1 in interstitial cells increased step by step, but collagen type Ⅳ decreased at first and then increased. CONCLUSION： AT-Ⅱ, macrophages and fibroblasts are all involved in pulmonary invalid remodeling course in early radiation pulmonary injuory, which is related to the initiation of pulmonary fibrosis in late period.
出处
《细胞与分子免疫学杂志》
CAS
CSCD
北大核心
2008年第2期119-121,共3页
Chinese Journal of Cellular and Molecular Immunology
基金
国家自然科学基金资助项目(30570545)
