摘要
建立刺嫩芽皂甙中齐墩果酸的含量测定方法。方法:采用HPLC,选用C18色谱柱,以乙腈-水(85:15)为流动相,流速为1.0mL/min,检测波长215nm。此方法在浓度为0.0125mg/mL～1.0000mg/mL范围内线性关系良好(R2=0.9995),平均回收率为101.7%,RSD=0.868%(n=6)。该色谱条件下齐墩果酸测定方法的专属性好,可用于刺嫩芽皂甙中齐墩果酸含量测定的方法学研究。
To determine the content of oleanolic in Araliaelata （Miq）Seem. HPLC was used. The HPLC system consisted of Hypersi 1C18（4.6 mm× 150 mm, 5 μm）column, Acetonitrile-water（85：15, v/v） was used for the mobile phase at a flow rate of 1.0 mL/min and the detective wave length was 215 nm. The linearity was good within the range of 0.012 5 mg/mL-1.000 0 mg/mL （R^2=0.999 5）.The average recovery was101.7 % and RSD was 0.868 % （n=6）.The chromatogram Conditions, which the determination method is specific and system suitability is satisfactory, can be used for determination the content of oleanolic acid in saponin ofA raliaelata （Miq）Seem.
出处
《食品研究与开发》
CAS
北大核心
2008年第2期114-116,共3页
Food Research and Development
基金
沈阳农业大学青年基金项目(2005039)
关键词
刺嫩芽
齐墩果酸
高效液相色谱法
含量测定
A raliaelata （Miq） Seem
oleanolic acid
HPLC
content determination
