摘要
目的探讨大鼠气管移植物经深低温冷冻保存后免疫原性降低的机制。方法Sprgue Dawley大鼠30只,取其气管冷冻保存于-85℃的冰箱。在新鲜和冷冻后1周、1个月、3个月、6个月共5个观察点上,采用苏木精-伊红染色来观察冷冻保存后气管黏膜上皮的形态改变,在透射电镜下观察DCs(dendritic cells,树突状细胞)的形态学改变,用免疫组化的方法分析冷冻保存后各个时间点气管上皮内的MHC-Ⅱ类分子的表达情况。结果随着冷冻保存时间的延长,大鼠气管黏膜上皮细胞脱落增多,MHC-Ⅱ类分子表达下调,同时黏膜上皮内DCs发生形态和功能方面的改变,表现为突起减少、线粒体基质深染及胞质内出现空泡变性等改变。结论大鼠气管冷冻后免疫原性降低可能有多种因素的参与,包括随着冷冻保存时间的延长出现的黏膜上皮脱落、MHC-Ⅱ类分子表达下调以及DCs发生形态和功能上的改变。
Objective To investigate the possible mechanism of depressed antigenicity of the cryopreserved trachea of rats. Methods Ten tracheal rings from thirty Sprague Dawley rats(fifteen male and fifteen female) were refrigerated at - 85℃ for 1 week,1 month,3 months,and 6 months. HE staining was performed to evaluate break off of epithelium of the cryopreserved trachea, and immuno- histo-chemistry was performed for the distribution of MHC- 11 in the epithelium of the rats' air way. Possible change of the distribution and shape of dendritic cells in the trachea were examined by transmission electron microscope. Results With the time of cryopreservation, we found more break off of epithelium of rats' trachea, less distribution of MHC-Ⅱ. For dendritic cells, severe injuries to the nucleus and important cellular organs such as mitochondria were discovered. Conclusions Many factors may contribute to the mechanism of depressed antigenicity of the cryopreserved trachea of rats, including the break off of the epithelium, alteration of distribution of MHC- Ⅱ and dendritic cells.
出处
《复旦学报(医学版)》
CAS
CSCD
北大核心
2008年第1期94-98,共5页
Fudan University Journal of Medical Sciences
基金
国家自然科学基金项目(30471628)