摘要
目的:探讨不同性状激光微孔脱细胞真皮构建活性基质的可行性。方法:将激光微孔猪脱细胞真皮分成两组即干燥组和湿润组,每组36片。将成纤维细胞悬液分别滴加在两组真皮表面,培养不同时间点后,每组分别随机留取6个孔的培养液,采用双抗体夹心ELISA法测定上清液中人TGF-β1、bFGF含量。结果:随着培养时间的延长,TGF-β1和bFGF分泌量逐渐增多,第8天达到稳定水平,第10、12天与第8天的含量比较差异无统计学意义(P>0.05);各时间点干燥组各因子的含量均高于湿润组(P<0.05)。结论:激光微孔猪脱细胞真皮可用为支架构建活性基质,其冷冻干燥后更有利于成纤维细胞的粘附与增殖。
Objective To investigate the feasibility of constructing active dermal matrix by inoculating fibroblasts on laser micropore porcine acelluar dermal matrix （PADM） in different physical characteristic. Methods Laser micropore PADM were divided into two groups, freeze-dry group and wet group, with 36 pieces in each groups. Human fibroblasts were inoculated on the surface of laser micropore PADM in two groups. The concentration of human TGF-131 and bFGF in the supernatant fluid of the culture medium were measured by ELISA method on 2, 4, 6, 8, 10, 12 days after inoculation. Results The concentration of TGF-131 and bFGF in two groups increased as the time of inoculation, and reached the peak value on 8 days after inoculation. The concentration on 10 days and 12days was not statistically different from that on 8 days after inoculation （P 〉 0.05）. The concentration in freeze-dry group was higher than that in wet group on each time point （P 〈 0.05）. Conclusion Active dermal matrix can be obtained by inoculating fibroblasts on the surface of laser micropore PADM, and the freeze-dried PADM is more suitable for adhesion and proliferation of fibroblasts.
出处
《中国美容医学》
CAS
2008年第1期66-68,共3页
Chinese Journal of Aesthetic Medicine
基金
全军医药卫生科研基金资助项目(06Z054)
北京市科技计划课题资助项目(H060920050830)
首都医学发展科研基金资助项目(2005-2040)
解放军总医院科技创新课题资助项目(06ZY29)
