摘要
用二氯甲烷-1mol／L醋酸钠溶液混合提取饲料中的三甲氧苄胺嘧啶，经离心分层后，取下层提取液用氮气吹干，1％（体积分数）冰醋酸复溶后用正己烷洗涤除脂两次，用液相色谱／紫外检测法测定，检测波长为270nm。结果表明，该方法平均回收率为80％～87％，相对标准偏差为3．0％～8．5％（n=4），配合饲料、浓缩饲料和预混合饲料中的检出限依次为1．2、2．0和0．9μg／g；定量下限依次为2．3、4．3和2．3μg／g。
Trimethoprim （TMP） was extracted from animal feeds by solution mixture of sodium acetate and dichloromethane, after being centrifuged, the low layer of extractant was separated and then evaporated to dryness by nitrogen flow, which was dissolved with 1% acetic acid, then defatted by liquid -liquid extraction（LLE） with hexane twice, finally determined by HPLC/UV at 270 nm. The mean recoveries of TMP ranged from 80% - 87% , with RSD 3.0% - 8.5% （ n = 4 ）, the limit of detection （LOD） 1.2 μg/g for compound feed, 2.0 μg/g for concentrated feed, and 0. 9 μg/g for premix feed, and the limit of quantitation（LOQ） 2.3,4.3 and 2. 3μg/g, respectively.
出处
《分析测试学报》
CAS
CSCD
北大核心
2008年第1期88-90,共3页
Journal of Instrumental Analysis
基金
国家标准项目(2000303-7-469)
