摘要
目的探讨雌激素对去卵巢大鼠生长发育、血液生化指标、骨营养状况及子宫增生的影响及作用机制。方法4月龄雌性SD大鼠腹腔假手术或切除双侧卵巢,分为假手术组(Sham)、去卵巢对照组(0vx)和雌激素组(EC)。用自行配制的含有β-雌二醇的饲料喂养7周,实验期间记录各组大鼠体重和摄食量,并计算食物利用率。实验结束后测定大鼠血清生化指标、股骨密度、骨钙含量,同时对子宫进行病理组织学观察。结果实验结束时雌激素组体重、食物利用率明显低于去卵巢对照组(P<0.01);雌激素组大鼠骨密度均明显高于去卵巢对照组(P<0.05);雌激素组大鼠子宫未见明显萎缩。结论雌激素可显著抑制大鼠去卵巢后体重增加,并有效减少骨量丢失,增强骨密度,对骨钙含量有增加趋势;同时对子宫的增生作用非常明显。
Objective To study the effects and mechanism of estrogen on growth and development, serum biochemical indexes, bone mass and uterus hyperplasia in ovariectomlzed rats that imitated postmenopausal women. Methods Four- month - old SD rats were sham - operated or ovariectomlzed by abdominal cavity operation and divided into sham, Ovx, and estrogen group（EC） and feed 7 weeks. During the experiment, the rats weight and food intake were recorded, the food utilization rates of each group were calculated. Serum biochemical indexes, bone mineral density（BMD）, calcium content and uterus hyperplasia were measured. Results The weight and food utilization rate of EC group decreased significantly（ P 〈 0.01 ）. The BMD of EC group was higher than Ovx group（P 〈0.05）. The uterus of EC group was not seen atrophy. Conclusion Estrogen could significantly inhibit Ovx induced weight gain, and were effective in preventing bone mass loss induced by ovariectomy and the uterus hyperplasia effection was also significantly.
出处
《中国公共卫生》
CAS
CSCD
北大核心
2008年第2期244-245,共2页
Chinese Journal of Public Health
基金
广东省自然科学基金(06012297)
广东省中医药局建设中医药强省科研课题(2060027)
关键词
雌激素
去卵巢大鼠
生长发育
骨代谢
estrogen, ovariectomlzed rats： growth and development
bone metabolism
