摘要
目的3种白色假丝酵母菌实验生物膜体外模型,观察生物膜形成的动态过程,比较3种模型研究结果的相关性。方法白色假丝酵母菌模式株ATCC 90038,分别用96孔板法,载玻片与6孔板法,以及连续培养法形成实验生物膜,荧光倒置显微镜观察生物膜的结构,XTT减低法定量检测生物膜的形成过程,比较3种方法形成的白色假丝酵母菌生物膜。结果3种方法都可以形成成熟的生物膜结构,定量分析有明显相关性。结论可以根据试验目的选择不同的白色假丝酵母菌实验生物膜的体外研究模型。
OBJECTIVE To compare the results of three kinds of Candida albicans biofilm model systems in vitro,and observe the dynamic course of C.albicans biofilm formation.METHODS C.albicans model strain ATCC 90038 formed biofilm structure on 96 well microtiter plates,on the glass plates in 6 well cell culture plates,and on the continuous culture systems-chemostat,which was observed by fluorescent microscope,and by XTT reduction method to quantitatively analyze the formation of biofilm.RESULTS All of the three kinds of biofilm model systems could form mature biofilm structure,and the quantitative analysis of biofilm formation indicated that three methods were significantly correlated.CONCLUSIONS These three model systems are all ideal methods for studying the C.albicans biofilm,we should choose the appropriate method according to objective of the study.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2007年第12期1478-1480,共3页
Chinese Journal of Nosocomiology
基金
国家自然科学基金资助项目(30400498)
