摘要
目的:探讨细胞色素P4502E1(CYP2E1)基因多态性,烟酒嗜好与哈萨克族食管癌易感性的关系.方法:采用1:2配比的病例对照研究方法,调查哈萨克族食管癌患者120例和非食管癌患者240例,采用聚合酶链-限制性片段长度多态(PCR-RFLP)方法检测CYP2E1 RsaⅠ位点的基因型.结果:病例组中CYP2E1 RsaⅠ位点C1/C1、C1/C2、C2/C2基因型频率与对照组比较(78.3%vs53.3%,19.2%vs 37.5%,2.5%vs 9.2%,X^2=21.794,P<0.01)差异有统计学意义:携带C1/C1基因型发生食管癌的危险性是携带C1/C2或C2/C2基因型的3.07倍(95%CI:1.87.5.03);交互作用提示CYP2E1基因多态与吸烟、饮酒均存在交互作用;其危险性远高于各单独作用之和.结论:CYP2E1 RsaⅠ位点基因多态性与大量吸烟、饮酒之间的基因-环境交互作用可增强哈萨克族人群患食管癌的风险.
AIM： To study the interaction between polymorphism in CYP2E1 genotype and tobacco and alcohol consumption and risk of developing esophageal cancer （EC） in Kazakh.
METHODS： A 1 ： 2 matched case-control study was conducted with 120 cases of EC in Kazakh and 240 population-based controls. CYP2E1 genotypes were identified by PCR amplification followed by digestion with Rsa Ⅰ.
RESULTS： The genotype frequencies of Rsa Ⅰ-digested CYP2E1 in the EC group differed from those in the control group （78.3% vs 53.3%, 19.2% vs 37.5%, 2.5% vs 9.2%, ;（2 = 21.794, P 〈 0.05）. Individuals with the C1/C1 genotype had a 3.07-fold （95% CI： 1.87 - 5.03） increased risk of developing EC compared with those who had the C1/C2 or C2/C2 genotype. Interaction analysis showed there was an interaction between CYP4502E1 gene polymorphism and smoking and alcohol consumption, and the interaction was higher than the sum of the two factors alone.
CONCLUSION： The gene-environment interaction between the Rsa Ⅰ -digested CYP2E1 polymorphism and smoking and alcohol consumption may increase susceptibility to EC in Kazakh.
出处
《世界华人消化杂志》
CAS
北大核心
2007年第36期3852-3855,共4页
World Chinese Journal of Digestology
基金
国家自然科学基金
No 30660161
国家重点基础研究发展规划资助项目(973计划)
No.2005CCA 03700
石河子大学博士基金
No.RCZX2004-PB17
2006年教育部科学技术研究重点资助项目
No.206167
