摘要
目的建立人血浆秋水仙碱检测的高效液相色谱-质谱检测方法,研究秋水仙碱片的人体药动学。方法血浆样品0.1 mL,经乙醚-二氯甲烷萃取,以ZORBAX Extend-C18为色谱柱;流动相为甲醇-10 mmol.L-1乙酸铵,流速为1.1 mL.min-1;采用质谱电喷雾离子化法,正离子多重反应检测(MRM)。测定12名健康志愿者单剂量口服2 mg秋水仙碱片后的血药浓度经时过程。由DAS2.0程序计算药动学参数。结果秋水仙碱浓度在0.05-10μg.L-1范围内线性关系良好;平均回收率分别为(92.47±1.73)%;日内RSD≤2.99%,日间RSD≤2.22%。结论本方法简便、快速、准确可靠,适用于人血浆秋水仙碱浓度的测定及其药代动力学研究。
Objective To develop a HPLC-MS/MS method for the determination of colchicine in human plasma, and to study its pharmacokinetics in healthy volunteers. Methods The plasma were extracted by Ethyl ether and dichloromethane. The analytical column was packed with ZORBAX Extend-C18. The mobile phase was Methanol-10 mmol· L^-1 ammonium acetate and the flow rate was 1.1 mL · min^-1. The selected ion was determined by ESI^＋. The colchicine concentrations of plasma were detected in 12 healthy volunteers at different time after they had been orally given 2 mg colchicines. The pharmacokinetic parameters were obtained with the DAS 2.0 program. Results Excellent liner relationship was obtained from the range of 0.05 μg · L^-1 to 10 μg · L^-1. The mean recovery rate was 92.47 ±1.73% , the RSDs of intra-and interday were less than 2.99% and 2.22% respectively. Conclusion The method is simple, rapid, accurate and can be used to determine the colchicine concentration in human plasma and to study its pharmacokinetics.
出处
《杭州师范学院学报（医学版）》
2007年第6期349-352,共4页
Journal of Hangzhou Teachers College :Medical Edition
