摘要
目的探讨超声破坏微泡造影剂刺激大鼠心肌血管内皮生长因子（VEGF）分泌、促进新血管生成的作用。方法建立大鼠心肌梗死模型，随机分为4组。A组行超声破坏微泡治疗，B组行单纯超声治疗，c组单纯注射微泡，D组注射生理盐水作为对照，每组均处理3次。4周后取材，免疫组化检测心肌组织中VEGF、CD34表达，ELISA及Western blot检测VEGF表达。结果A组心肌组织中见大量VEGF和CD34表达，新生血管较多；B组有较少VEGF和CD34表达，新生血管较少；C组和D组仅有极少量VEGF和CD34表达，未见明显新生血管。ELISA检测显示，A组VEGF表达量明显增高[（8．194±1．129）pg／g]，与其他三组[B组（4．339±0．753）pg／g，C组（2．304±0．762）pg／g，D组（2．411±0．801）Pg／g]比较，差异均有统计学意义（P〈0．05）。Westernblot检测显示A组有一条约21kD的明显的蛋白质条带表达。结论超声破坏微泡可刺激心肌内源性VEGF分泌，促进血管新生。
Objective To investigate whether ultrasound-mediated microbubble destruction can stimulate secretion of VEGF and angiogenesis in myocardium of rat. Methods Forty normal Wistar rats were randomly devided into 4 groups,each group was successed raised myocardial infarction model. In group A, the myocardium was exposed to ultrasound after intravenous injection of microbubble of 1.0 ml; in group B, ultrasound was simply used in the myocardium;in group C, microbubble was intravenous injection only; in the control group （group D）, normal saline was infused. Each group was received 3 experiments. Immunohistochemical staining of VEGF and CD34 were performed, and the quantitate of VEGF was detected by ELISA 4 weeks later. Western blot was performed for expression of VEGF. Results Immunohistochemical staining showed that the expressions of VEGF and CD34 were higher than those in group B, C, D;and the new vessels degeneration were significant observed in group A than those in group B, C, D. ELISA detection showed that the quantities of VEGF were siginificant different in groups（ P 〈0.05）, it was highest in group A. A protein strap about 21 kD was shown in group A by Western blot detection,a tenuous protein strap about 21 kD was shown respectively in the other three groups. Conclusions Ultrasound-mediated microbubble destruction can facilitate the endogenous secretion of VEGF and accelerate the vascularization in the rat myocardium.
出处
《中华超声影像学杂志》
CSCD
2007年第12期1071-1073,共3页
Chinese Journal of Ultrasonography
基金
国家高技术研究发展计划（863计划）项目（21）06AA0224F0）
2006年国家教育部高等学校博士学科点专项科研基金（20060631009）
重庆市卫生局科研面上项目（05-2-015）
关键词
超声疗法
造影剂
心肌梗塞
新生血管化
病理性
Ultrasonic therapy
Contrast media
Myocardial infarction
Neovascularization, pathologic
