摘要
本实验以酵母β-1,3-葡聚糖为原料,利用木霉菌株LE02产β-1,3-葡聚糖酶对其进行酶解增溶,并对酶解产物组分进行了分析,以得到最大量的可溶性多糖。结果表明,最佳酶解条件为55℃、pH4.5、底物浓度20%、酶用量4U/ml,酶解时间2h,可溶性多糖得率达到52%;不同水解时间酶解产物经SephadexG-100凝胶过滤均可得到组分I(大分子量多糖)、组分II(寡聚糖)和组分III(小分子量低聚糖和单糖)三个组分,随着水解时间延长组分Ⅰ比例下降、而组分Ⅲ的比例迅速上升。凝胶渗透色谱(GPC)测定水解2h酶解产物的分子量分布,其中分子量大于30kD组分占总糖的比例为66.2%。
Yeast β-1,3-glucan was hydrolyzed by the β-1,3-glucanase with Trichoderma strain LE02. The conditions of enzymatic hydrolysis were optimized to improve the solubility of amyloses and obtain the maximum water soluble amylose, while the hydrolysate fractions were analyzed by GPC（gel-permeafion chromatography）. The maximum yield of water soluble β-1,3-glucan is 52% when 20% substrates with 4 U/ml enzyme are incubated at 55 ℃ in pH4.5 buffer for 2 h. The hydrolysates separated through Sephadex G-100 include three fractions： high-molecular-weightpolysaccharides（Fraction Ⅰ）, oligosaccharides （Fraction Ⅱ）, and low-molecular-weight oligosaccharides （Fraction Ⅲ）. The ratio of Fraction I decreases while Fraction Ⅲ increases rapidly as the β-1,3-glucan was hydrolyzed. The molecular weight distribution of the hydrolysates hydrolyzed for 2 h is analyzed by GPC and over 66.2% fraction is of the molecular weight larger than 30 kD.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2008年第1期185-189,共5页
Food Science
基金
湖北省重大科技攻关计划项目(2006AA204A03)
