摘要
为确定河南地区杨树黄叶病的病因并提出有效的防治措施,开展了元素补充、组织粉碎回接、微生物分离回接、线虫检测、电子显微镜切片观察、植原体的PCR及DAPI检测等实验。结果表明:对轻度病株施硫酸亚铁有较好的治疗效果;回接组织粉碎物的健株无症状表现;从病株的根、茎、叶中分离了4种微生物并进行了回接,27.5%的接种苗出现了与黄叶病相似的症状;在病株体内没有发现植原体和内寄生性线虫;电子显微镜下观测到了杆状细菌存在。研究结果对该病害的后续研究具有重要的参考价值。
In order to ascertain the pathogeny of poplar yellow-leaf disease from Henan province and take effective treated-measure, element mending, separated microorganisms inoculation, endoparasites detection, electron microscope slice up observation, PCR and DAPI of phytoplasma experiments were developed in this paper. The light diseased trees could be better after being sprinkled FeSO4. Four kinds of microorganisms were separated and the inoculation indicated 27.5% poplar seeding showed yellow-leaf-alike symptom. Phytoplasma and endoparasites did not exist in the diseased trees. Abundant bacilli were observed under scanning electron microscope. These results have important referenced value for future investigation of this disease.
出处
《中国农学通报》
CSCD
2008年第1期384-388,共5页
Chinese Agricultural Science Bulletin
基金
河南省重点科技攻关项目"杨树黄叶病害致病机理及预防控制技术研究"072102150004)
关键词
杨树
黄叶病
症状
发病规律
病原
poplar, yellow-leaf disease, symptom, occurrence law, pathogeny
