摘要
建立HPLC法测定小儿感冒颗粒中连翘苷的含量。以Kromasil C18为色谱柱,流动相：乙腈-水（25∶75）,流速：1.0mL·min^-1,检测波长：277nm,线性范围在0.10-1.05μg（r=0.9999）。平均加样回收率99.9%（n=5）,RSD=1.25%。本法简便快捷,结果准确,重复性好,分离效果好。
A HPLC method was established for the determination of forsythin in Xiaoerganmao Granules.Kromasil C18column was used with a mobile phase of Acetonitrile-water（25∶75）.The content was detected at a wavelength of 277nm.The flow rate was 1.0mL.min-1.The calibration curve was linear in the range of 0.10 - 1.05μg（r= 0.9999）.The average recover was 99.9% and RSD = 1.25%.The method is highly reproducible and accurate.
出处
《中国药事》
CAS
2008年第1期59-60,共2页
Chinese Pharmaceutical Affairs
