摘要
目的建立宁夏枸杞黄酮类化合物高效液相色谱指纹图谱,为鉴别不同来源的宁夏枸杞提供依据。方法以10个宁夏不同产地的宁夏枸杞主栽品种"宁杞号"样品建立枸杞黄酮类化合物指纹图谱共有模式,采用"中药色谱指纹图谱相似度评价系统"软件进行数据处理,对15个不同来源的枸杞样品进行了分析。结果本研究建立的方法重现性较好;16个特征峰构成了宁夏枸杞黄酮类化合物色谱指纹图谱的特征;不同产地、不同品种的枸杞样品黄酮类化合物组成与量存在差异。结论所建立的枸杞黄酮类化合物的HPLC指纹图谱对不同产地、不同品种枸杞的鉴别具有参考价值。
Objective To establish chromatograph by HPLC and distinguish the Fructus Lycii from diff ic fingerprint of flavonoids from Lycium barbarurn erent habitats. Methods The mutual mode was established depending on ten Fructus Lycii samples from different growing areas in Ningxia. The solftware "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica" was applied to analyzing 15 Fructus Lycii samples from different habitats. Results The established chromatographic fingerprint method had good repeatability; 16 peaks are characteristic of HPLC fingerprints of flavonoids and flavonoids of Fructus Lycii from different habitats were quite different in content and constituent. Conclusion This chromatographic fingerprint spectrum of flavonoids could be used to distinguish Fructus Lycii from different habitats.
出处
《中草药》
CAS
CSCD
北大核心
2008年第1期103-105,共3页
Chinese Traditional and Herbal Drugs
基金
教育部科学技术项目(204148)
宁夏大学自然科学基金项目
宁夏自然基金重大项目(2003ZD01)