摘要
目的黄曲霉毒素B1快速检测技术的研究。方法以甲基丙烯酸为改性剂,苯乙烯为单体,采用无皂乳液聚合法制备了羧化聚苯乙烯微球(CPLP)。以黄曲霉毒B1肟(AFB1O)与牛血清白蛋白(BSA)的连接物(AFB1O-BSA)致敏CPLP,用于黄曲霉毒素B1(AFB1)快速检测。结果制备的AFB1O-BSA致敏CPLP胶乳试剂,最低检出量可达5ng/ml。结论本研究制备的快速检测胶乳,具有微量、简便、快速和特异性强的特点,可用于AFB1的定性和半定量检测,适宜现场快速检测。
Objective To study on rapied detection technique of aflatoxin B1. Methods Carboxylated polystyrene latex particles（CPLP） were prepared by soap-free polymerization using styrene and a-methyl crylic acid. The CPLP were sensitized with aflatoxin B1 oxime-BSA for the detection of aflatoxin B1 . Results The minimum detect limits of sensitized latex were 5ng/ml, Conclusion The sensitized latex could applied to detect qualitative and half-quantitative aflatoxin Bi in fieldwork. The technique maybe minim, simpleness, rapidness and specificity.
出处
《卫生研究》
CAS
CSCD
北大核心
2008年第1期98-100,共3页
Journal of Hygiene Research
关键词
羧化聚苯乙烯微球
致敏
黄曲霉毒B1
检测技术
carboxylated polystyrene latex particles, sensitize, aflatoxin B1 , detect technique
