摘要
目的：研究不同含锌饲料对幼鼠脑金属硫蛋白（Ｍｅｔａｌｏｔｈｉｏｎｅｉｎ，ＭＴ）表达的影响。方法：从哺乳期开始建立幼鼠低锌（ＺｉｎｃＤｅｆｉｃｉｅｎｃｙ，ＺＤ）、常锌（ＺｉｎｃＮｏｒｍａｌ，ＺＮ）和高锌（ＺｉｎｃＥｘｃｅｓｓｉｖｅ，ＺＥ）动物模型，以流式细胞仪及免疫组化的方法，定位、定量观测脑组织（皮层、海马）内ＭＴ表达的变化。结果：幼鼠在哺乳期末（４周）和７周末脑组织内ＭＴ含量：ＺＤ组明显低于ＺＮ组，而ＺＥ组明显高于ＺＮ组。ＭＴ广泛分布于胶质细胞，散在分布于神经元内，在脉络膜、脑室管膜细胞及血管内皮细胞内含量丰富。结论：饲料锌明显影响幼鼠脑ＭＴ表达。ＭＴ可能对脑组织细胞内锌的代谢及生物学功能起重要调节作用，与脑发育有关。
Objective: To investigate the effects of different zinc diets on the expression of metallothionein(MT) in the young rat brain.Methods: After the zinc deficient (ZD), zinc normal (ZN) and zinc excessive (ZE) models were established in young rats, the changes of MT expression in the brain tissues (cortex and hippocrampus) were quantitatively and locationally observed with flow cytometry and immunohistochemistry. Results: At the end of 4 and 7 weeks, the content of MT was significantly lower in ZD group but significantly higher in ZE group than in ZN group. MT was widely distributed in glial cells and loosely in neurons. A large amount of MT was seen in choroid plexus epithelium, ependymal cells and endothelial cells of the blood vessels. Conclusion: Dietary zinc obviously affects MT expression in the brain of young rats. MT may exert important regulative effects on the metabolism of tissue zinc and biological function of zinc and is associated with the development of the brain of young rats.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
1997年第4期352-355,共4页
Journal of Third Military Medical University
关键词
锌
金属硫蛋白
脑
幼鼠
zinc
metallothionein
brain
immunohistochemistry
rat
