摘要
目的探讨压力对体外培养的猪眼小梁细胞基质金属蛋白酶-2(matrix metallo-proteinases,MMP-2)、MMP-3以及基质金属蛋白酶组织抑制剂-1(tissue inhibitors of MMPs,TIMP-1)表达的影响。方法培养猪眼小梁细胞并进行鉴定。对传第3代的小梁细胞施加20mmHg(1kPa=7.5mmHg)、40mmHg、60mmHg及80mmHg压力作为实验组,0mm-Hg设为对照组。培养48h后对MMP-2、MMP-3和TIMP-1进行免疫组织化学SP法染色及电镜扫描,并对染色结果进行统计学分析。结果根据培养细胞的生长特性、形态特征及细胞免疫组织化学染色结果等特点,确定培养的细胞为猪眼小梁细胞。猪眼小梁细胞正常可以少量表达MMP-2、MMP-3和TIMP-1。20mmHg、40mmHg、60mmHg时猪眼小梁细胞MMP-2的SP染色细胞阳性率分别为(34.30±10.89)%、(47.48±4.96)%、(58.40±8.67)%,TIMP-1的SP染色细胞阳性率分别为(20.40±7.63)%、(37.66±11.64)%、(49.58±8.10)%,MMP-3的SP染色细胞阳性率分别为(12.30±4.35)%、(11.98±2.26)%、(15.24±5.63)%.因此压力改变在一定程度上可以促进猪眼小梁细胞表达MMP-2、TIMP-1,而对猪眼小梁细胞MMP-3的表达无明显影响。结论一定范围内压力的变化可以改变MMPs/TIMPs之间的平衡状态,进而影响小梁细胞外基质细胞外基质的代谢以及房水外流阻力,因此MMPs在青光眼的发病机制中发挥重要作用。
Objective To investigate the effect of pressure on the expression of matrix metalloproteinase-2 （MMP-2）, MMP-3 and tissue inhtbitiors of MMPs-1 （TIMP-1） in the cultured swine trabecular cells. Methods Swine meshwork cells were cultured and identified. Cultured trabecttlar meshwork cells were treated with 20 mmHg（ 1 kea =7.5 mmHg） ,40 mmHg,60 mmHg and 80 mmHg pressure respectively in the treatment group,and no pressure was given in the control group. Tmmunoeytoehemical staining and electron microscope scanning were used to observe the expression of MMP-2 ,MMP-3 and TIMP-1 after eultured 48 hours. The data were statistically analyzed. Results The eultttred cells were identitied as the trabecular cells for their growth eharacters, morphologic eharacters and cell immunoeytochemiCal data. The cells can MMP-2 ,MMP-3 and TIMP-1 at small quantity. When R were 20 mmHg,40 mmH and 60 mmHg, the cell sleuthed positive rate of MMP-2 were （ 34. 30 ± 10. 89）% ,（47.48 ±4.96）% and （58.40 ±8.67）% ,respectively;The cell positive rate of TIMP-1 were（20.40 ±7.63）% ,（37.66 ± 11.64）% and（49.58 ±8.10）% and the cell positive rate of MMP-3 were（12.30 ±4.35）% ,（11.98 ±2.26）% and（15.24 ± 5. 63 ） % , respectively. With the pressure raising, the expression of MMP-2 and TIMP-1 were increasing,but the expression of MMP-3 had no significant variation. Conclusion The raising pressure can induce the increasing expression of MMP-2,TIMP-1 on the cultured swine trabecular cells,and affect the bAlAnce of extracellular matrix,which contribute to the pathogenesis of glaucoma. [ Rec Adv Ophthalmoi 2008 ;28 （2） ： 116-118,133 ]
出处
《眼科新进展》
CAS
2008年第2期116-118,133,共4页
Recent Advances in Ophthalmology
关键词
压力
小梁细胞
青光眼
基质金属蛋白酶
pressure
trabeculat cell
glaucoma
matrix metalloprotetnnses
