摘要
目的观察U50488H预处理及低温保存对离体兔心的保护作用。方法将40只大白兔均分为5组,每组8只。通过Langendorff装置建立离体心脏灌注模型,对照组:不用药物进行预处理,用UW液保存心脏6h;组:用含U50488H(1.6mmol/L)的St.Thomas心脏停搏液预处理,离体心脏低温保存4h;组:预处理同组,低温保存6h;组:预处理同组,低温保存8h;组:预处理同组,低温保存10h。离体心脏在再灌注30min后检测心功能、心肌肌浆网钙离子三磷酸腺苷酶(SRCa2+-ATPase)活性和心肌线粒体Ca2+浓度。结果随着离体心脏低温保存时间的延长,心功能各项指标的恢复率、冠状动脉流量(Cf)的恢复率和SRCa2+-ATPase的活性呈下降趋势,而线粒体Ca2+浓度随着心脏低温保存时间的延长而逐渐升高。组和组上述心功能指标恢复率分别高于组和组(P<0.05,0.01),组恢复率高于组(P<0.05)。组Cf的恢复率(84.56%±10.38%)高于组(79.45%±9.67%)、组(68.31%±6.84%,P<0.01),组高于组(P<0.05)。组SRCa2+-ATPase的活性(4.43±0.41μmol/mg.h)分别高于对照组(3.04±0.22μmol/mg.h)、组(3.26±0.29μmol/mg.h)和组(2.57±0.63μmol/mg.h,P<0.05),组高于组(P<0.01)。组线粒体Ca2+浓度(38.76±4.30μmol/g.dw)和对照组(40.23±3.75μmol/g.dw)分别低于组(43.25±5.16μmol/g.dw)和组(45.78±3.26μmol/g.dw,P<0.05,0.01)。结论U50488H预处理及U50488H保存液对离体供心的低温保存时间应控制在8h以内;UW液对离体心脏的保存作用与U50488H预处理及低温保存6h效果相当。
Objective To observe the combined protective effects of U50 488H and hypothermia preservation on isolated rabbit hearts preconditioned. Methods Forty rabbits were randomly divided into five groups, 8 rabbits in each group. The perfusion model of isolated rabbit hearts was established by the Langendorff device. In the control group： the isolated rabbit hearts were preserved with the University of Wissconsin solution （UW） for six hours; group Ⅰ：the isolated rabbit hearts were preconditioned with St. Thomas Ⅱ cardioplegic solution containing U50 488H （1.6mmol/L）and then preserved with hypothermic preservation for four hours; group Ⅱ; the precondition was the same as group Ⅰ, hypothermic preservation for six hours; group Ⅲ： the precondition was the same as group Ⅰ, hypothermic preservation for eight hours; group Ⅳ： the precondition was the same as group Ⅰ, hypothermic preservation for ten hours. The cardiac function, myocardial sarcoplasmic reticulum calcium ion adenosine triphosphatase （SRCa^2＋-ATPase） activity and calcium ion concentrations in mitochondria were determined at thirty minutes after reperfusion. Results As the hypothermic preservation time increased from four to ten hours, the recovery rate of each index of cardiac function,coronary artery flow（Cf） and SRCa^2＋-ATPase activity also decreased, but the calcium ion concentrations in the mitochondria increased. Cardiac function index recovery rates in group Ⅰ and group Ⅱ were higher than those in group Ⅲ and group Ⅳ respectively（P〈0. 05,0. 01）,meanwhile recovery rates of cardiac function index in group Ⅲ were higher than that in group Ⅳ （P〈0.05）. Recovery rate of Cf in group Ⅱ （84. 56%±10. 38%）were higher than those in group Ⅲ （79.45%±9. 67%）and group Ⅳ （68. 31%±6. 84%,P〈 0. 01）, meanwhile the recovery rate of Cf in group Ⅲ was higher than that in group Ⅳ （P〈0.05）. SRCa^2＋-ATPase activity in group Ⅱ （4.43±0.41μmol/mg·h）were higher than those in control group（3.04±0. 22μmol/mg·h）, group Ⅲ （3.26±0. 29μmol/mg·h） and group Ⅳ （2.57±0. 63μmol/mg·h,P〈0. 05）, SRCa^2＋-ATPase activity in group Ⅲ was higher than that in group Ⅳ （P〈0. 01）. The calcium ion concentrations in mitochondria in group Ⅱ （38.76±4. 30μmol/g·dw）and in the control group （40. 23±3. 75μmol/g·dw）were less than those in group Ⅲ （43.25±5.16μmol/g·dw）and group Ⅳ （45.78±3.26μmol/g·dw,P〈0. 05,0. 01）respectively. Conclusion The hypothermic preservation time for isolated donor＇s hearts pre-treated with St. Thomas Ⅱ cardioplegic solution containing US0 488H should the kept under 8h. The myocardial protection effects of both UW solution and US0 488H- containing St. Thomas Ⅱ cardioplegic solution on isolated donor＇s hearts appear to be the same at 6 hours.
出处
《中国胸心血管外科临床杂志》
CAS
2008年第1期44-47,共4页
Chinese Journal of Clinical Thoracic and Cardiovascular Surgery
