摘要
目的研究间充质干细胞（MSC）与其诱导的血管内皮细胞（Ec）联合种植于多孔β-磷酸三钙（β-TCP）构筑血管化组织工程骨修复大段骨缺损的作用。方法制备兔双侧尺骨1．5cm骨缺损共64侧，分4组修复（n=16），A空白未治疗组，B单纯材料组（植入β-TCP），C组织工程骨组（植入MSC＋β-TCP），D血管化组织工程骨组（植入MSC＋EC＋β-TCP），各组交叉配对。术后4、8、12、16周行X线片、放射性核素骨显像、大体解剖、组织切片、生物力学等检查，观察各组骨缺损修复效能及移植物血管化情况。结果D血管化组织工程骨组完美修复骨缺损，且修复效能及血管化情况优于C组织工程骨组，C组织工程骨组优于B单纯材料组，A空白组未能修复骨缺损。各组结果差异有统计学意义（均P〈0.05）。结论MSC与其诱导EC联合种植构筑的血管化组织工程骨能促进成骨过程和新生骨的血管化，显著提高组织工程骨修复大段骨缺损的能力。
Objective To study the ability to repair large segmental bone defects of vascularized bone of tissue engineering constructed by co-seeding mesenchymal stem cells （MSCs） and endothelial cells （ECs） induced from the MSCs in porous β-tricalcium phosphate （TCP）, Methods Rabbit MSCs were obtained, cultured, and induced to differentiate into ECs. 1, 5 cm ulna diaphyseal defects were made bilaterally in 32 rabbits, The 64 bone defects were randomly divided into 4 equal groups Group A： without treatment, Group B, implanted with β-TCP, Group C, implanted with tissue-engineered bone （ MSCs ＋ 6- TCP） , and Group D, implanted with vascularized bone of tissue engineering （ MSCs ＋ ECs ＋ β-TCP）. Xray and ECT examination, HE staining of tissue slices of the implants, and biomechanical test of the specimens were carried out 4, 8, 12, and 16 weeks postoperatively to evaluate the repairing effect of the bone defects and vascularization of the implants. Results The bone defects of Group A showed little trace of osteogenesis. The bone defects of Group D showed a more perfect repairing effect and vascularization of implants than Group C. Conclusion The vascularized bone of tissue engineering constructed by MSCs and ECs induced from MSCs co-seeded in porous β-TCP can accelerate the vascularization and osteogenesis process of the implants, and is more effective in repairing large segmental bone defects.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2008年第5期337-341,共5页
National Medical Journal of China
基金
国家自然科学基金资助项目（30271262）
上海市卫生局科技发展基金资助项目（054076）
教育部博士点基金资助项目（20060246074）
上海市优秀学科带头人计划基金资助项目（07XD14006）
关键词
干细胞
组织疗法
骨生成
Stem cells
Tissue therapy
Osteogenesis
