摘要
目的探讨母体孕期染铝对子代脑神经发育的毒性作用。方法4～5月龄sD大鼠，雌雄各30只，1：1合笼喂养，每日早晨查雌鼠阴栓，发现阴栓即进入实验（E0），将孕鼠随机分为两组。染铝组E0-E18期间以AlCl3 100mg/（kg·d）灌胃；正常对照组以等量蒸馏水灌胃。分别取孕鼠三个年龄段子代即18d胚胎、生后继续喂养的1月子代及3月子代鼠海马组织，每只孕鼠任取其子代2只，每小组子代10只。常规石蜡切片，行胶质纤维酸性蛋白（GFAP）免疫组化染色，计数海马CA2区GFAP免疫组化染色阳性反应细胞，并用细胞形态学计量方法测量各阳性反应产物的平均光密度值。结果铝中毒组子代GFAP阳性细胞明显多于对照组（P〈0．05），阳性反应产物平均光密度明显高于对照组（P〈0．05）。结论大鼠孕期染铝可导致子代海马CA2区星形胶质细胞增多；从而导致子代鼠神经发育障碍。
Objective To investigate the developmental neurotoxicity of filial generation in aluminum exposure to pregnant rats, and to observe the aluminium effect on astrocytes of offspring in aluminium exposure to pregnant rats. nethotis 30 male and 30 female SD rats of 4 - 5 months age, the rats were mated, check up female rats＇ vaginal plug in the morning at every day , the day for discovering vaginal plug is E0. Pregnant rats were randomly divided into two equal groups： the aluminum exposed group （pregnant rats were given AlCl3 100 mg/kg/d by digestive tract from E0 to E18 ）, and normal control group （pregnant rats were gaved given equal distilled water）. The offsprings were divided into 3 groups： 18 days embryo group, 1 month group and 3 month group. After traditional paraffin section preparation, the brain were stained immunohistoneactively, and GFAP-positive ceils were quantitatively analyzed with cell morphometric technique. Results The numbers of GFAP positive cells in the pexiform layer of hippocampal CA2 areas were obviously increased in aluminum exposed group than that of normal control group, their average opiticaloptical density were obviously higher, respectively. Conclusion The numbers of astrocytes are increased in aluminum exposure to pregnant rats, which may be caused by that aluminum lead to developmental neurotoxicity.
出处
《山东医药》
CAS
北大核心
2008年第2期24-26,共3页
Shandong Medical Journal
基金
贵州省科学技术基金资助项目(黔科合计(2004)3107号)
关键词
孕期
铝
脑
免疫组化
大鼠子代
duration of pregnancy
aluminium
brain
immunohistochemistry
offspring
