摘要
目的采用毛细管气相色谱法测定哮喘胶囊中吗啡的含量，建立哮喘胶囊质量控制体系。方法以邻苯二甲酸二正壬酯（DNP）为内标，石英毛细管柱SE-54（30m×0．32mm×0．32μm）为固定相，氮气为载气，FID检测器。结果在该色谱条件下，吗啡在0．080～0．801mg·ml^-1的浓度范围内线性关系良好（r〉0．9995）；平均回收率为99．4％，RSD为2．6％。结论该方法简单、灵敏、快速、回收率和重现性好，可以作为哮喘胶囊的质量控制标准。
Objective To establish a GC method for determination of Morphine in Xiaochuan Capsules by gas chromatography. Methods DNP was used as the internal standard substance . The GC system consisted of Capillary column, SE - 54 （30 m × 0.32 mm × 0.32μm）as the stationary phase, nitrogen as the carrier gas, and FID as the detector. Results Morphine had a good linearity within the range of 0. 080 - 0.801 mg · ml ^-1 under the chromatographic condition; the mean recovery of morphine was 99.4%, and RSD was 2.6%. Conclusion The method is simple, sensitive and rapid with satisfactory recoveries and reproducibilities.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2008年第1期46-48,共3页
Lishizhen Medicine and Materia Medica Research
基金
贵州省中药现代化专项(No.[2004]32)
关键词
毛细管气相色谱法
哮喘胶囊
吗啡
Capillary gas chromatography
Xiaochuan capsules
Morphine
