摘要
目的探讨更简易和合理有效的前列腺增生(BPH)动物模型的建立方法,并对比分析其不同特点。方法选用40只BALB/C小鼠,随机分为对照组(去势睾酮组)和实验组(非去势睾酮组),两组均为20只。对照组先作双侧睾丸切除术,而实验组则做同一切口但不切除睾丸。1周后,两组小鼠都同时给予丙酸睾酮(12.5mg/kg·d-1)腹腔内连续注射20d,第21天所有小鼠均采血处死。解剖观察前列腺特点并称重,做病理检查,血清作睾酮、雌二醇和血管内皮生长因子检测。结果两组小鼠BPH模型均建立成功。实验组前列腺增生较对照组更明显(P<0.05),呈单侧腹侧叶结节状非对称性增生,与临床BPH发病机制和病理相比,对照组更接近。结论小鼠不经去势而使用睾酮原液腹腔内注射,可获更为简易和合理的BPH模型。
Objective To search a simpler, better and more reasonable benign prostatic hyperplasia （BPH） animal model. Methods 40 BALB/C mice were randomly and equally distributed into 2 groups： castrated mice as control group, whereas non-castrated mice as study group. The mice in castrated group were suffered with bilateral orchiectomies whereas those in non-castrated groups were only suffered with sham operations. One week later, all mice in two groups were injected through abdominal cavities with testosterone stock solutions as 12.5mg/kg·rat^-1·d^-1 for consecutive 20 days. All mice were sacrificed at the 21 st day. Their ventral and dorsal prostates were observed and weighed individually and were studied by pathological skills, while their serums were studied for T, E2, VEGF analyses. Results Larger and higher hyperplasia and more pink secreting fluids were found in ventral prostatic glands in non-castrated mice than those in castrated ones, meanwhile pathological features in non：castrated group showed more similarities to clinical BPH patients. Six detail improvements were proposed in this article for BPH model establishment. Conclusion Non-castrated mice with low-dose and short-term testosterone would probably procure an easier and more feasible and reasonable BPH animal model.
出处
《中国男科学杂志》
CAS
CSCD
2008年第1期24-27,共4页
Chinese Journal of Andrology
关键词
小鼠
前列腺增生
模型
动物
mice
prostatic hyperplasia
models, animal
