摘要
目的 了解国内健康人和器官移植受者体内人类白细胞抗原-GS（HLA-G5）的表达情况，并探索其表达的时间规律。方法 分别采集30例健康人（健康查体正常）、50例肝移植受者（肝移植术后3个月且肝功能稳定）、50例肾移植受者（肾移植术后3个月且肾功能稳定）的外周血各3ml，并采集33例肝肾移植受者术前及术后1周、4周、12周、1年的外周血各3ml。提取血清，采用酶联免疫法（ELISA）检测HLAG5水平。结果 30例健康人中，28例HLAG5的A值低于0．5，根据标准曲线含量为0．0ng／ml，2例分别为8、9ng／ml。50例肝移植受者HLA-G5表达阳性者16例，阳性率为32％，其中4例含量高于30ng／ml。50例肾移植受者HLA-G5表达阳性者10例，阳性率为20％，有1例含量为25ng／ml。健康人、肝移植、肾移植3组血清中HLA-G5平均含量分别为0．56±0．20、8．34±1．50和3．26±0．25ng／ml。对33例肝肾移植受者进行动态检测显示，有1例患者术前和术后1周HLA-G5呈阳性，4例术后4周阳性，12例术后12周阳性，追踪至术后1年有11例阳性。结论 HLA-G5在健康人体内低表达，与器官移植免疫耐受有一定相关性。在移植后移植物接受良好的情况下，HLA-G5有不同水平的表达，肝移植受者的HLA-G5表达量高于肾移植受者。33例肝肾移植受者的动态结果证实了HLA-G5的表达从mRNA转录到蛋白表达的时间为15-60d的观点，HLAG5的表达在第60天到达高峰，并具有稳定性。
Objective To investigate the expression of human leukocyte antigen-G5 （HLA-G5） in healthy Chinese people and the recipients of renal and liver transplantation. The regulating mechanism of the expression of HLA-G5 was discussed by comparing the ex- pression of HLA-G5 in the healthy people with that in renal and liver transplantation recipients. Furthermore, the changing regularity with time was studied by kinesis supervising the expression of HLAG5 in renal and liver transplantation recipients. Methods The peripheral blood samples （3ml） from 30 health people, 50 recipients of liver transplantation （liver function was stable 3 months after liver transplanta tion） and 50 recipients of renal transplantation （renal function was stable 3 months after renal transplantation） were collected. Peripheral blood samples were also collected in same amount from 33 recipients of renal and liver transplantation before operation and 1, 4 and 12 weeks and 1 year after operation. The HLA-G5 of all serum samples was analyzed by ELISA. Results For 30 healthy people, the OD vat ue of HLA G5 in 28 people was below 0. 5, for which the contents were defined as 0. 0ng/ml according to standard and the contents for the other 2 people were 8ng/ml and 9ng/ml, respectively. 16 of 50 recipients undergone liver transplantation were positive for the expression of HLA G5, the positive ratio was 32%. The contents in 4 recipients were higher than 30ng/ml. 10 of 50 recipients of renal transplantation were positive in the expression of HLA-G5, the positive ratio was 20%. The contents in one recipient were higher than 25ng/ml. The av erage contents in sera of healthy people, recipients of liver or renal transplantation were 0. 56±0. 20ng/ml, 8. 34±1.50ng/ml and 3. 26±0. 25ng/ml, respectively. For 33 recipients of liver or renal transplantation, the expression of HLA-G5 was detected by ELISA, and it was found that one recipient the expression of HLA-G5 was positive before operation and within 1 week after operation; expression of HLA-G5 was positive in 4 recipients within 4 weeks after operation; expression of HLA G5 was positive within 12 weeks after operation in 12 recipients; and the expression of HLA-G5 was positive within 1 year after operation in 11 recipients. Condusion The expression of HLAG5 in healthy people is low. There are correlation between the expression of HLA-G5 and immunotolerance to transplants. In minor rejection condition after transplantation, there are different expression levels of HLA-G5, and it is higher after liver transplantation than renal transplantation. The time for expression of HLA-G5 corresponds with the time for mRNA of HLA-G5 transcription into protein, and it is about 15-60 days, with 60 days as the peak time.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2008年第1期20-22,共3页
Medical Journal of Chinese People's Liberation Army
关键词
HLA抗原
肝移植
肾移植
HLA antigens
liver transplantation
kidney transplantation
