摘要
目的观察表皮生长因子受体(EGFR)在结直肠癌原发灶及转移灶中的表达情况。方法采用免疫组织化学法检测37例(男25例,女12例)术后出现远处转移的结直肠癌患者原发灶及转移灶EGFR的表达情况,其中结肠癌16例(43.2%),直肠癌21例(56.8%),肝转移32例(86.5%),腹股沟淋巴结转移5例(13.5%)。EGFR阳性细胞≥5%即判定为阳性。结果37例中有25例(67.6%)原发灶EGFR阳性,其中7例(28.0%)对应的转移灶EGFR阴性。12例原发灶EGFR阴性者中,有3例(25.0%)对应的转移灶EGFR阳性。统计分析表明,原发灶与转移灶EGFR的表达无统计学差异(P=0.23)。结论转移性结直肠癌原发灶与转移灶的EGFR表达状态无显著性差异,其中大部分病例表达情况一致。
Objective Epidermal growth factor receptor （EGFR） plays an important role in the pathogenesis of colorectal cancer （CRC）. There are several potential therapies to target EGFR The detection of EGFR expression is usually performed by immunohistochemistry （IHC） only in the primary tumor. There are a few data regarding the EGFR status in the corresponding distant metastases. The aim of the present study was to correlate EGFR expression in primary tumors and related metastases in order to find out whether EGFR status is different between primary tumors and related metastases. Methods 37 cases （25 male, 12 female） of metastatic colorectal cancer were collected. The site of primary tumor was on colon in 16 patients （43. 2%） and on rectum in 21 patients （56. 8%）. Metastatic sites analyzed were liver in 32 patients （86. 5%）, inguinal lymph nodes in 5 patients （13.5%）. The EGFR status of prirrkary tumors and related metastatic sites were evaluated retrospectively. The expression of EGFR was analyzed by immunohistochemical staining （IHC）, EGFR status was defined as positive if the percentage of stained malignant cells was ~5%, and the expression feature was analyzed. Results EGFR status was positive in 25 primary tumors （67. 6%）, in which the corresponding metastatic site was negative in 7 eases （28. 0%）, whereas it was posi tive in 3 metastases （25. 0%） from 12 cases of EGFR-negafive primary cancers. The difference between the priruary tumors and metastatic lesions had no statistic significance （P=0. 23）. Conclusion When analyzed by IHC, The difference of EGFR status has no statistic significance between the paired primary tumors and distant metastatic lesions, and 73% of samples show concordant EGFR status
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2008年第1期80-81,共2页
Medical Journal of Chinese People's Liberation Army
关键词
结直肠肿瘤
受体
表皮生长因子
肿瘤转移
colorectal neoplasms
receptor, epidermal growth factor
neoplasm metastasis
