摘要
利用AFLP和SRAP标记对19株毛木耳进行遗传多样性分析。12对AFLP引物扩增得到624条片段,14对SRAP引物扩增得到459条片段,采用聚类分析都供试材料被分为4个群。结果表明,AFLP和SRAP标记均适合毛木耳遗传多样性分析,而SRAP标记较AFLP标记简便,更适于大规模的遗传多样性分析。
Due to unsatisfying attempts to fingerprint A. polytricha, two different molecular maker systems, AFLP and SRAP, were established and tested to quantify molecular diversity among 19 strains of this fungus. A total of 624 and 459 polymorphic bands were detected by 12 AFLP primers and 14 SRAP primer combinations, respectively. By parsimony method, a phylogenetic tree was constructed based on each analysis. And the two trees showed that 19 A. polytricha strains were distributed into four groups, respectively. These results demonstrated that both methods were suitable for the discriminating among strains of A. polytricha, and the novel SRAP markers are more efficient and preferable. The result also indicated that the high level of genetic diversity of A. polytricha and their relationship between each others. These finds would be benefit to future research in A. polytricha, especially in breeding and medicine development. It also gave a useful method for other fungi fingertyping.
出处
《西南农业学报》
CSCD
2008年第1期121-124,共4页
Southwest China Journal of Agricultural Sciences
基金
四川省育种攻关项目
