摘要
目的探讨曲马多预先给药对切口痛大鼠背根神经节神经生长因子（NGF）表达的影响。方法雄性SD大鼠24只，体重200～250g，随机分为4组（n=6），对照组（C组）仅给予麻醉及腹腔注射生理盐水5ml；切口痛组（1组）于术前30min腹腔注射生理盐水5ml；曲马多1mg／kg预先给药组（T1组）和曲马多10mg／kg预先给药组（T10组）分别于术前30min腹腔注射曲马多1、10mg／kg。制备大鼠右后爪切口痛模型，T1组和T10组大鼠苏醒后采用累积疼痛评分法评定痛行为学，于术前30min和术后2h测定大鼠机械痛阈、热痛阈及右侧背根神经节NGF的表达水平。结果与C组比较，1组、T1组术后2h机械痛阈和热痛阈降低，累积疼痛评分升高，背根神经节NGF表达上调，T10组背根神经节NGF表达上调（P〈0．05或0．01）；与1组比较，T1组和T10组术后2h机械痛阈和热痛阈升高，累积疼痛评分降低，背根神经节NGF表达下调（P〈0．05）；与T1组比较，T10组术后2h机械痛阈和热痛阈升高，累积疼痛评分降低（P〈0．05或0．01），背根神经节NGF表达差异无统计学意义（P〉0．05）。结论曲马多1、10mg／kg腹腔内预先给药均可下调切口痛大鼠背根神经节NGF表达水平。
Objective To investigate the effects of tramadol pretreatment on the expression of nerve growth factor （NGF） in the dorsal root ganglion （DRG） of the spinal cord in a rat model of incisional pain. Methods Twenty-four adult male SD rats weighing 200-250 g were randomly divided into 4 groups （ n = 6 each） ： group Ⅰ control （C）; groupⅡ incisional pain （ I ）; group Ⅲ and Ⅳ tramadol pretreatment 1 and 2 （T1, T2 ）. In groupⅡ , Ⅲ and Ⅳ an lcm incision was made in the plantar surface of the right hind-paw, according to the method described by Brennan. Group C received intraperitoneal （IP） normal saline （NS） 5 ml ; group Ⅰ received NS 5 ml IP at 30 min before operation; group T1 and T2 received tramadol 1 and 10 mg/kg IP respectively at 30 min before operation. Paw-withdrawal threshold to von Frey filament stimuli and thermal stimuli were measured at 30 min before surgery and 2 h after operation. Cumulative pain score was calculated based on the behavior of the ipsilateral paw of the rats （0 = touch the floor with body weight, 2 = does not touch the floor） at 1 h after the rats emerged from anesthesia, and pain scores were recorded every 5 min for 60 min. The expression of NGF in the ipsilateral DRG was determined by immuno-histochemistry. Results Plantar incision significantly decreased the mechanical and thermal pain threshold and increased cumulative pain score. Tramadol pretreatment significantly reduced incisional pain in a dose-dependent manner in group Ⅲ and Ⅳ as compared with group Ⅱ . NGF expression in the ispilateral DRG was significantly higher in group Ⅱ , Ⅲ and Ⅳ than in control group and was significantly lower in the two tramaadol pretreatment groups （ group Ⅲ and Ⅳ ） than in group Ⅱ . ConclusionPretreatment with intraperitoneal tramadol 1 and 10 mg/kg can inhibit the expression of NGF in the ipsilateral DRG of the spinal cord in a rat model of incisional pain.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2008年第1期53-56,共4页
Chinese Journal of Anesthesiology
