摘要
A new system of FRET was built in this paper.In the condition of λex/λem=480nm/530nm,0.2mol/L HCl,the effective energy transfer can occur between Eosin Y(EY)and Rhodamine B(Rhb).The quenching of fluorescence of the system happened with the addition of BSA.According to that a new determination of BSA was developed.The quenching of EY-RhB is linear with BSA from 10mg/L to 40mg/L,and the limit of determination is 9.8mg/L.
A new system of FRET was built in this paper. In the condition of λex/λem = 480nm/530nm , 0. 2mol/L HCl, the effective energy transfer can occur between Eosin Y（EY） and Rhodamine B（Rhb）. The quenching of fluorescence of the system happened with the addition of BSA. According to that a new determination of BSA was developed. The quenching of EY - RhB is linear with BSA from 10mg/L to 40mg/L,and the limit of determination is 9. 8mg/L
出处
《化学研究与应用》
CAS
CSCD
北大核心
2008年第2期181-183,共3页
Chemical Research and Application
关键词
曙红Y
罗丹明B
牛血清白蛋白
荧光共振能量转移
Eosin Y
Rhodamine B
Bovine Serum Albumin
Fluorescence Resonance Energy Transfer
