摘要
目的:研究姜黄素对人前列腺增生细胞BPH1增殖及基因表达的抑制。方法:MTT法测定姜黄素抗增殖效应;逆转录-聚合酶链反应(RT-PCR)技术检测细胞凋亡抑制基因-2(Bcl-2)和增殖细胞核抗原(PCNA)mRNA的表达。结果:各实验组的姜黄素对增生细胞的生长均有抑制,抑制作用具有时间/剂量依赖性;姜黄素作用于BPH1细胞24h,Bcl-2和PCNA mRNA的表达水平下降,呈剂量依赖性。结论:姜黄素可抑制人良性前列腺增生细胞BPH1的增殖,其机制可能与姜黄素下调Bcl-2和PCNA mRNA的表达有关。
OBJECTIVE： To investigate the inhibitory effect of Curcumine on the proliferation and gene expression of human prostatic hyperplasia cell line BPH1. METHODS： MTT were used to examine the cell growth of Human prostatic hyperplasia cell line BPH1 after treated with different doses of Curcumine. The expressions of PCNA mRNA and Bcl - 2 at transcription were detected by RT-PCR. RESULTS： Curcumine inhibited cell growth time or dose- dependently and downregulated the expressions of PCNA and Bcl- 2 in BPH1 cells dose-dependently within 24 h after being treated by Curcumin.CONCLUSION： The antiproliferative effect of Curcumine on BPH1 might be associated to the downregulation of PCNA and Bcl - 2 levels.
出处
《中国药房》
CAS
CSCD
北大核心
2008年第6期404-406,共3页
China Pharmacy
基金
国家自然科学基金资助项目(30360124)
关键词
姜黄素
前列腺增生
凋亡
Curcumin
Prostatic hyperplasia
Apoposis
