摘要
目的:研究凉血化瘀法对实验性自身免疫性甲状腺炎(experi mental autoi mmune thyroiditis,EAT)模型大鼠的疗效及对其外周血单核细胞肿瘤坏死因子α和白细胞介素-10的影响。方法:建立大鼠EAT模型,同时设立正常大鼠对照组。将EAT模型大鼠随机分成模型组、环孢素A组、雷公藤多苷组及中药凉血化瘀方组,给予灌胃治疗4周后,处死大鼠,观察甲状腺组织病理改变和检测血清中抗甲状腺球蛋白抗体(thyroglobulin antibodies,TgAb)水平;实时荧光定量逆转录-聚合酶链式反应(real-ti me reverse transcription polymerase chain reaction,real-ti me RT-PCR)法研究大鼠外周血单核细胞肿瘤坏死因子α(tumor necrosis factor-alpha,TNF-α)和白细胞介素-10(interleukin-10,IL-10)mRNA的表达情况。结果:模型组TgAb水平明显升高并出现甲状腺炎症,且模型组外周血单核细胞TNF-αmRNA表达水平升高,IL-10mRNA水平下降(P<0.01)。与模型组相比,各治疗组TgAb水平均降低(P<0.01),甲状腺炎症严重程度无明显改善(P>0.05),各治疗组外周血单核细胞TNF-αmRNA水平下降,IL-10mRNA水平升高(P<0.05)。结论:凉血化瘀法除能下调EAT模型大鼠TgAb水平外,还可改善EAT大鼠细胞因子Th1/Th2平衡,为进一步研究用EAT模型作为评价抗银屑病中药药效的代替模型提供了实验基础。
Objective：To explore the effects of Liangxue Huayu therapy（LXHYT）,a traditional Chinese herbal therapy for cooling blood and dissipating blood stasis,on rat model of experimental autoimmune thyroiditis（EAT）and its impact on tumor necrosis factor-alpha and interleukin-10 mRNA expressions in peripheral blood mononuclear cells.Methods：Forty Wistar rats were randomly divided into five groups：normal control group,untreated EAT group,cyclosporine A（CyA）-treated group,Tripterygium glycosides-treated group and Liangxue Huayu Recipe（LXHYR）-treated group.The interventions were given by gavage to the rats in different groups once a day.All rats were sacrificed after 4-week treatment,and the level of serum thyroglobulin antibodies（TgAb）and the changes of histological grade of thyroid specimen were assessed by blind evaluation.Expressions of tumor necrosis factor-alpha（TNF-α）and interleukin-10（IL-10）mRNAs in peripheral blood mononuclear cells were assessed by real-time reverse transcription polymerase chain reaction method.Results：There was a significant increase in the serum TgAb level and severe inflammatory infiltration in the untreated group.Expression of TNF-α mRNA in peripheral blood mononuclear cells was increased,while the expression of IL-10 mRNA was decreased in the untreated group（P〈0.01）.Compared with the untreated group,CyA,Tripterygium glycosides and LXHYF could decrease the serum TgAb level（P〈0.05）,but the three interventions showed no significant improvement in thyroid inflammation（P〉0.05）.TNF-α mRNA expression was decreased,while IL-10 mRNA expression was increased as compared with the untreated group（P〈0.05）.Conclusion：LXHYT can decrease the serum TgAb level and recover the balance of Th1/Th2.This may provide an experimental basis for further research of assessing the antipsoriatic effect of Chinese herbal drugs with a rat model of EAT as an alternative model of psoriasis in vivo.
出处
《中西医结合学报》
CAS
2008年第2期190-193,共4页
Journal of Chinese Integrative Medicine
