摘要
目的:筛选最佳的白术种子生活力和发芽率测定方法,明确生活力与发芽率的相关性。方法:以12个白术地方品种为试验材料,采用四唑染色法、红墨水染色法、BTB法、农家简易法测定种子生活力,用种子发芽试验测定发芽率。结果:四唑染色法测定白术种子生活力的结果是4种方法中最高的,它与发芽率的相关系数(r)为0.915,也比其他3种方法高得多,并且达到了显著水平,进一步求得四唑法测定的生活力(X)与发芽率(Y)回归方程为Y=-0.083 4+0.995 4X。结论:四唑染色法是测定白术种子生活力的最佳方法,其测得的生活力与发芽率呈显著的正相关。
Objective： To screen the optimized methods for detection seed viability and germination rate determination of Atractylodes macrocephala, and determine the relationship between seed viability and germination rate. Method： There were four methods, which including 2,3,5-triphynel tetrazolilum chloride （TTC） staining, red ink staining, BTB staining and Nongjia method, to evaluate the 12 A. macrocephala local varieties＇ seed viability and measure their germination rate. Result： Seed viability of A. macocephala using TTC staining ranked the first compared to that of other three methods. Seed viability was significantly related with germination rate using TTC method. Their correlation coefficient reached 0. 915 and regression equation was also found out between seed viability （X） and germination rate （ Y）, which was Y = - 0. 083 4 ＋ 0. 995 4X. Conclusion： TTC staining was the optimal method to determine A. macrocephala seed vitality. Furthermore, seed viability was significant related with germination rate of A. macrocephala.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2008年第3期248-250,共3页
China Journal of Chinese Materia Medica
基金
国家科技攻关计划项目(2001BA701A59)
关键词
药用植物
白术
生活力
发芽率
相关性
medical plants
Atractylodes macrocephala
viability
germination rate
correlation
