摘要
目的:观察慢性粒细胞白血病(CML)初发和急变患者外周血BCR/ABL融合基因和转录因子FoxO3a的表达变化及其意义。方法:利用EVA Green建立实时荧光RT-PCR检测BCR/ABL、FoxO3a和GAPDH的方法,检测25例CML初发和25例CML急变患者的外周血样本中BCR/ABL融合基因和FoxO3a的表达,以及30例正常人外周血样本中FoxO3a的表达,以GAPDH为内参基因,各组基因表达水平的差异采用△△CT相对定量法。结果:CML急变患者外周血BCR/ABL融合基因的表达水平是初发患者的4.72倍,正常人外周血样本中FoxO3a的表达水平分别是CML初发和急变患者的143.39倍和8.18倍。结论:BCR/ABL融合基因的高表达与转录因子FoxO3a的低表达可能与CML不同阶段的病理过程相关联。
Objective：To observe the expression of BCR/ABL fusion gene and FoxO3a transcription factor in the patients with primaiy or crisis blast CML leukemia. Methods：Using EVA Green,real-time RT-PCR methods for the detection of BCR/ABL,FoxO3a and GAPDH were explored. BCR/ABL and FoxO3a gene in 25 PBMC samples with primary CML leukemia and that of 25 cases with crisis blast CML leukemia were detected by real-time RT-PCR and 30 cases of health adults were also detected for FoxO3a gene. The difference of those genes expression between the groups was analyzed by AACt relative quantity means, in which GAPDH gene was used as control. Results ： The expression of BCR/ABL fusion gene in the patients with crisis blast leukemia was 4,72 times higher than that of the patients with primary CML leukemia.For the expression of FoxO3a gene, the group of health adults was 143.39 and 8.18 times higher than those of primary and crisis blast leukemia group,respectively. Conclusion：Over-expression of BCR/ABL fusion gene and low-expression of FoxO3a were possibly correlated with the pathology process of CML in different phase.
出处
《南京医科大学学报（自然科学版）》
CAS
CSCD
北大核心
2008年第2期190-193,共4页
Journal of Nanjing Medical University(Natural Sciences)
基金
南京医科大学科技发展重点项目(2005NY-DZD35)
