摘要
目的：研究大黄对肠粘膜及肠血管通透性的影响。方法：选用低血容量性休克和内毒素性休克大鼠动物模型，以荧光标记白蛋白和小肠湿／干重比值检测内毒素性休克肠血管通透性，以血浆内毒素含量来衡量肠粘膜通透性。结果：内毒素能引起小肠组织明显水肿，其湿／干重比值显著增高，同时明显提高肠血管壁对荧光标记白蛋白的通透性；而大黄能减轻肠壁水肿和湿／干重比值（内毒素组为３．７５±０．６８，大黄组为１．６６±０．３３，Ｐ＜０．０１），降低肠血管通透性〔小肠组织荧光标记白蛋白含量：内毒素组为（１．２５４±０．１１７）μｍｏｌ／ｇ，大黄组为（０．９００±０．０７１）μｍｏｌ／ｇ，Ｐ＜０．０１〕。低血容量性休克能破坏肠粘膜屏障，提高肠粘膜对内毒素的通透性，而大黄可明显降低低血容量性休克大鼠肠粘膜通透性，抑制肠道内毒素的吸收〔血浆内毒素含量：休克组为（０．５５７±０．０６９）ＥＵ／ｍｌ，大黄组为（０．３４５±０．０５５）ＥＵ／ｍｌ，Ｐ＜０．０１〕。结论：大黄能保护肠粘膜屏障，抑制肠道内毒素吸收，降低肠粘膜及肠毛细血管通透性。
Objective:To investigate the effect of rhubarb on permeability changes in intestinal mucosa and capillary.Methods:Animal models of hemorrhagic and endotoxic shock were used in the present study.The wet/dry ratio and fluorescein isothiocyanate labeling albumin were used to measure the permeability of intestinal capillary in rats after endotoxic shock,and plasma endotoxin concentration was used to monitor the intestinal mucosa permeability in rats after hemorrhagic shock.Results:Endotoxin challenge could obviously induce intestinal edema and increase intestinal vascular permeability,whereas rhubarb treatment could markedly alleviate intestinal edema (wet/dry ratio:endotoxin group vs.rhubarb group:3 75±0 68 vs. 1 66±0 33, P <0 01),and decrease intestinal vascular permeability fluorescein isothiocyanate labeling albumin content:endotoxin group vs. rhubarb group:(1 254±0 117) vs.(0 900±0 071)μmol/g, P <0 01 .Similarly,hemorrhagic shock resulted in gut barrier dysfunction and promoted endotoxin absorption from the gut,but rhubarb treatment inhibited endotoxin absorption plasma endotoxin concentration:hemorrhagic shock group vs. rhubarb group:(0 557±0 069) vs.(0 345±0 055)EU/ml, P <0 01 .Conclusions:It is suggested that rhubarb may have protective effect on gut barrier,inhibit endotoxin absorption from the gut,and decrease permeability changes in intestinal mucosa and capillary.
出处
《中国危重病急救医学》
CAS
CSCD
1997年第7期385-387,共3页
Chinese Critical Care Medicine
基金
国家自然科学基金
关键词
大黄
休克
内毒素
肠粘膜
药理
rhubarb
shock
endotoxin
intestinal mucosa
