摘要
将γ-生育酚甲基转移酶基因导入油菜,旨在提高菜油中的维生素E含量。为了使该基因在油菜种子中特异表达,采用油菜种子特异启动子BcNA1,构建了植物表达载体pBIBE。以花油五号和花油六号油菜品种子叶柄为受体,将γ-生育酚甲基转移酶基因(γ-TMT)通过农杆菌介导法转化油菜,获得了22株抗卡那霉素再生植株。经过PCR检测,其中有7株表现为阳性,初步证明γ-TMT已整合到油菜基因组中。
The plant expression vector pBIBE was constructed by inserting γ-TMT gene and BcNA1 promoter into the plant high-efficient expression vector pBI121. The cotyledon handle from Brassica napus L. cultivars Huayou No. 5 and Huayou No. 6 were transformed by Agrobacterium-mediated method with expression vector pBI121. Twenty-two kanamycin resistant plants were examined by PCR with specific primers. Seven plants showed positive signal, indicating that γ- TMT was transformed into the genome of these seven plants.
出处
《激光生物学报》
CAS
CSCD
2008年第1期86-89,共4页
Acta Laser Biology Sinica
