摘要
目的观察灯盏细辛注射液对大鼠肾间质纤维化的改善作用并探讨其机制。方法采用单侧输尿管梗阻(unilateral ureteral obstruction,UUO)大鼠模型为研究对象。36只SD大鼠随机分为对照组、UUO模型组(模型组)和灯盏细辛治疗组(治疗组)。治疗组每天给予灯盏细辛注射液〔5mL/(kg.d)〕腹腔注射治疗,由术前24h始至术后第9天止;术后第10天取术侧肾脏组织,常规石蜡包埋切片,HE、PAS和苦味酸-天狼星红染色,光镜下观察大鼠肾小管间质病理改变、肾脏皮质相对间质容积和相对胶原含量的变化;免疫组织化学方法检测肾脏转化生长因子β1(TGF-β1)、α-平滑肌肌动蛋白(α-SMA)和Ⅰ型胶原的表达。结果模型组和治疗组均出现明显的肾间质纤维化病理改变,但治疗组病变程度相对较轻。肾皮质相对间质容积和相对胶原含量模型组及治疗组明显高于对照组,但治疗组明显少于模型组(P<0.01)。免疫组化染色显示:TGF-β1、α-SMA和Ⅰ型胶原表达模型组和治疗组明显多于对照组,但治疗组较模型组明显减少(P<0.05)。结论灯盏细辛注射液对大鼠肾间质纤维化具有显著的改善作用,这种作用可能是通过抑制TGF-β1表达,阻止肾小管上皮细胞转分化,减少Ⅰ型胶原合成实现的。
Objective To investigate the effect and mechanism of Erigeron Injection （EI） on renal interstitial fibrosis in rats. Methods Unilateral ureteral obstruction （UUO） model rats were taken as the subject of study. Thirty-six Sprague-Dawley rats were randomly divided into the control group （A）, the UUO model group （B） and the treatment group （C） treated with intraperitoneal injection of EI 5 mL/kg per day from 24 h before to 9 days after the operation. On the 10th day of UUO, rats were killed and their kidneys were processed to paraffin sections with HE, PAS and picro-sirius-red staining. The pathological change of renal tubular interstitial tissue and relative cortical/interstitial volume （C/I） as well as the relative content of collagen （RC） were observed by light microscope. The expression of transforming growth factor β1 （TGF-β1）, α-smooth muscle actin （α-SMA） and collagen Ⅰ in the renal mesenchyma were examined by immunohistochemistry. Results Marked renal interstitial fibrosis changes were found in Group B and C, but the changes were milder in Group C. C/I and RC were higher in Groups B and C as compared with those in Group A （P 〈0.01 ）, but they were much lower in Group C than in Group B （P 〈 0. 01 ）. The expression of TGF-β1 , α-SMA and collagen Ⅰ were higher in Group B and C than those in Group A （P 〈 0. 05 ）, but they were lower in Group C than in Group B （ P 〈 0. 05 ）. Conclusion EI could ameliorate renal interstitial fibrosis in rats, which might be partially realized by down-regulating the expression of TGF-β1 to prevent the renal epithelial cell differentiation and reducing the synthesis of collagen Ⅰ .
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2008年第2期142-145,共4页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
灯盏细辛
肾间质纤维化
转化生长因子Β1
Ⅰ型胶原
Erigeron Injection
renal interstitial fibrosis
transforming growth factor-β1
collagen Ⅰ
