摘要
背景与目的:survivin是一种凋亡抑制基因,在肿瘤发生和转归中起重要作用。本研究探讨survivin基因启动子区域-31G/C多态性与胃癌及survivinmRNA表达的关系。方法:采用聚合酶链反应-限制性片段长度多态性分析技术(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)检测96例胃癌患者癌组织和67例正常人外周血中的survivin基因多态性,同时用实时定量逆转录聚合酶链反应(real-time quantitative reverse transcription-polymerase chain reaction,RQ-RT-PCR)对肿瘤组织的survivin mRNA进行了定量检测。结果:胃癌患者survivin-31位GG、GC和CC基因型频率为20.84%、39.58%和39.58%,而正常对照组则分别为46.26%、41.80%和11.94%。胃癌患者-31C等位基因和C/C基因型频率分别为59.37%和39.58%,显著高于对照组的32.84%和11.94%[χ2=22.26,P<0.005,OR=2.98,95%CI=1.96～4.51;χ2=14.88,P<0.005,OR=4.83,95%CI=2.91～8.03]。胃癌组织高表达survivin mRNA,但其表达强度在各基因型之间无显著性差异。结论:survivin基因启动子-31C/C基因型和C等位基因与胃癌有关,可能是肿瘤发生的易感因素之一。
BACKGROUND ＆ OBJECTIVE： Survivin, a bifunctional protein that regulates cell division and suppresses apoptosis, may play an important role in tumorigenesis. This study was to determine the correlations of survivin gene 31-GC polymorphisms to the occurrence of gastric carcinoma and survivin mRNA expression. METHODS： The -31G/C single nucleotide polymorphism （SNP） of survivin promoter in peripheral blood samples from 96 gastric carcinoma patients and 67 healthy subjects was analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR- RFLP） and gene sequencing. Survivin mRNA expression in gastric carcinoma tissues was detected by real-time quantitative reverse transcriptionpolymerase chain reaction （RQ-RT-PCR）. RESULTS： The genotype frequencies for -31G/G, -31G/C and -31C/C were 20.84%, 39.58% and 39.58% in gastric carcinoma patients, and 46.26%, 41.80% and 11.94% in healthy subjects, respectively. The frequencies of survivin-31C allele and C/ C genotype were significantly higher in gastric carcinoma patients than in healthy subjects [59.37% vs. 32.84%, χ^2 = 22.26, P〈0.005, odds ratio （OR）=2.98, 95% confidence interval （CI）=1.96-4.51; 39.58% vs. 11.94%, χ^2 =14.88, P〈0.005, OR=4.83, 95% CI=2.91-8.03], but survivin mRNA was overexpressed with no significant difference in gastric cancer tissues subgrouped by genotypes. CONCLUSION： The -31C genotype of survivin promoter is associated with gastric cancer, and may be a risk factor of gastric carcinoma.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2008年第3期258-263,共6页
Chinese Journal of Cancer
