摘要
在已建立的滨梅组织培养快繁的基础上,为了进一步提高滨梅繁殖的效率与质量,在培养基中附加TDZ和NaCl,其有效促进了滨梅不定芽的快速诱导与增殖。在添加质量浓度为1.0mg/L的TDZ时,可显著提高不定芽的增殖数,每个外植体增殖的芽数达(37.4±4.93)个,但茎高只有(0.80±0.07)cm,显著低于其他质量浓度TDZ(0.05,0.10,0.50mg/L)的处理;0.5mg/LTDZ处理的外植体,其芽增殖数为(26.6±3.59)个,与不加TDZ相比,差异显著,该处理的茎高则为(1.42±0.11)cm,与不加TDZ比较,没有显著差异;培养基MS+ZT2.0mg/L+BSA(牛血清白蛋白)30mg/L+TDZ0.5mg/L对滨梅不定芽的增殖与生长是适宜的。在此基础上,于培养基中附加0.1%NaCl能更有效地提高不定芽的增殖,每个外植体芽增殖数为(29.83±1.74)个,与附加0和0.3%的NaCl相比,差异显著。TDZ和NaCl组合对滨梅快速繁殖是有效的,其适宜培养基是MS+ZT2.0mg/L+BSA30mg/L+TDZ0.5mg/L+0.1%NaCl。经过壮苗培养后,在生根培养基诱导的根质量良好,有效地提高了小苗的成活率。
Based on the previous research on in vitro culture of beach plum, our work was carried out further on the in vitro culture of beach plum. It proves that the media containing TDZ and NaCl are more efficient in promoting regeneration of beach plum than those chosen before. The highest regeneration efficiency （37.4 ± 4.93 shoots per explant） was observed on the medium supplemented with 1.0 mg/L TDZ, but the shoot height is the lowest, only （0.80 ± 0.07） cm. The number of ad- ventitious shoots and the shoot height were （26.6 ±_ 3.59） and （ 1.42 ±_ 0.11 ） cm per explant for the treatment with 0.5 mg/L TDZ. The difference in shoot number is more significant and in shoot height is unobvious compared with those of the control （ without TDZ）. The optimal medium for the in vitro regeneration of beach plum is MS medium containing 2.0 mg/ L ZT, 30 mg/L bovine serum albumin （BSA） and 0.5 mg/L TDZ. Moreover, the medium containing 0.1% NaCl is bene- ficial to the multiplication of adventitious shoots, and the shoot number （29.83 ± 1.74 shoots per explant） is significantly higher than that of the medium with 0.03% NaCl or without NaCl. The combination of TDZ and NaCl is effective to the rapid reproduction of beach plum, and the suitable medium is MS with ZT 2.0 mg/L, BSA 30 mg/L, TDZ 0.5 mg/L and 0.1% NaCl. The shoots are well induced on the rooting medium, which results in a higher survival rate.
出处
《东北林业大学学报》
CAS
CSCD
北大核心
2008年第2期8-9,22,共3页
Journal of Northeast Forestry University
基金
国家"十一五"科技支撑计划(2006BAD09A04
2006BAD09A08)
