摘要
目的比较不同孔径的细胞复合型丝素支架材料体内成骨、修复兔桡骨骨缺损的治疗效果。方法自新西兰大耳白兔股骨大结节和股骨髁处抽取骨髓，体外分离、培养、扩增兔骨髓间充质干细胞（rabbitbone marrow mesenchymal stem cells，RBMSCs），接种到3种不同孔径的丝素材料上，再移植于兔桡骨骨缺损处。根据丝素材料孔径设立实验A组[RBMSCs／SF（silk fibroin，SF）-Ⅰ]、实验B组（RBMSCs／SF-Ⅱ）、实验c组（RBMSCs／SFⅢ）。术后分别于2、4、8、12周大体观察、X线检查及组织形态学观察了解体内成骨、骨缺损修复及材料降解情况。另外设立二对照组，D组为缺损处仅植入丝素支架，E组为缺损处无植入物。结果术后各组兔子均存活，无植入物毒性反应及排斥反应，材料体积不同程度降解减小，降解速度SF-Ⅰ〈SF-Ⅱ〈SF-Ⅲ。 RBMSCs／SF-Ⅱ组比其他组在缺损种植处有更多的新骨形成，移植物的血管化在此B组也更好，缺损被较满意治愈。D、E二组修复骨缺损的效果明显差于细胞型丝素支架。结论RBMSCs／SF-Ⅱ作为细胞复合型丝素支架材料表现最佳。细胞复合型丝素支架材料有望用于临床骨缺损的治疗。
Objective To explore the. impacts of pore size of bone marrow mesenchyrnal stem cells seeded in silk fibroin （SF） scaffold on the repair of radial bone defect in rabbits. Methods The animal model of 15 mm long metaphyseal unibilateral radius defect was created in 40 New Zealand white rabbits, and randomly assigned into five groups： In group A （n = 8）, the radial bone defect was repaired with RBMSCs/SF- Ⅰ （SF with 58 μm pore size） coroposites implantation, group B （n = 8） with RBMSCs/SF-Ⅱ （SF with 78 lain pore size） composites, group C （n = 8） with RBMSCs/SF-Ⅲ （SF with Ⅲ μm pore size） composites, group D SF-Ⅱ without seeding cells, and group E without any implantation as controls. At the end of 2, 4, 8, and 12 weeks after implantation, a series of examina tions including radiograph and histomorphology were carried out to analyze the formation of ossicula and the degradation of graft. Results All rabbits survived after operation, and no reactions of trans plantation toxicity or rejection were observed. Degenerative rates of grafts could be summarized as SF-Ⅰ〈SF-Ⅱ〈SF-Ⅲ. The volume of the graft progressively decreased and grafts were degenerated in vivo in all three groups. There was more new bone formed in the defective bone areas in group B than that in the other two groups, and grafts were vascularized more broadly in group B, eventually defects were healed with osteal reconstruction. Conclusions RBMSCs/SF- Ⅱ composites are the best for the repair of radial bone defects in rabbit, BMSCs and silk fibroin composites may be used as a prospective biomedical material for clinical bone defect reconstruction in the future.
出处
《中华小儿外科杂志》
CSCD
北大核心
2008年第1期44-48,共5页
Chinese Journal of Pediatric Surgery
基金
江苏省卫生厅135工程课题（38RC2002038）
苏州市科技发展规划课题（SSY0630）
