摘要
目的应用层层静电自组装技术构建一种新的精氨酸-甘氨酸-天冬氨酸(RGD)固定方法,并与物理吸附和化学偶联法进行比较。方法55片10mm×10mm×1mmTi-6Al-4V片经喷砂、双重酸和过氧化氢热处理后,30片应用层层静电自组装技术固定RGD,并与物理吸附、应用CDI的化学偶联技术固定RGD相比较。场发射扫描电镜(FSEM)观察RGD固定过程中钛片表面形貌情况,荧光显微镜大体观察固定到钛片上的RGD数量。结果FSEM显示物理吸附和新的固定方法不会破坏钛片的表面形貌,而应用CDI的化学偶联则破坏钛片原有的表面形貌,大小不等的多极孔洞被完全破坏。荧光显微镜图片显示物理吸附固定到钛片表面的RGD较少,而应用CDI的化学偶联和新的固定方法固定到钛片表面的RGD较多。结论采用层层静电自组装技术有利于将RGD固定到钛表面且不破坏种植体表面形貌,同时可保证数量和结合强度。
Objective To establish a new method to immobilize RGD onto porous-titanium surface using the layer-by-layer electrostatic self- assembly technique, and compare it with the physical adsorption and one of covalent coupling techniques. Methods 55 Ti-6Al-4V discs of 10 mm× 10 mm× 1 mm used in this study were in turn treated by sandblasting, HF/HNO3 solution,HCl/H2SO4 solution,and H2O2/HCl solution. Physical adsorption,covalent coupling using CDI and a new method using the layer-by-layer electrostatic serf-assembly technique were used to immobilize the RGD sequence onto the titanium surfaces. The morphology changes of the disc surfaces were observed by field-emissing scanning electron microscopy（FSEM）. The peptide GRGDSP was labeled by fluorescein isothiocyanate （FITC） as the fluorescent probes in order to detect the peptide immobilized onto the Ti-6Al-4V surface. Results FSEM micrograph showed that the physical adsorption and the new method did not destroy the disc surfaces. In contrast, the covalent coupling using CDI destroyed the disc surfaces. The multilevel and different-sized holes were entirely destroyed. Fluorescence micrographs showed there was less peptide grafted to the surface using physical adsorption. The peptide grafted to the surface using covalent coupling and the new method was more. Conclusions The new method using layer-by-layer electrostatic self-assembly technique is propitious to immobilize the RGD sequence onto the titanium surface without destroying the surface. The method ensured the quantity and combined strength of RGD.
出处
《口腔医学》
CAS
2008年第2期61-64,74,共5页
Stomatology
基金
浙江省科技计划重点资助项目(2005C23006)
