摘要
目的:探讨间接原位PCR技术在弓形虫淋巴结炎病理诊断中的应用。方法:设计弓形虫B1基因1对引物进行原位扩增,选择弓形虫B1基因来源的地高辛标记探针进行扩增后原位杂交,应用间接原位PCR技术,检测三所医院共86例病理诊断为慢性非特异性淋巴结炎石蜡包埋切片组织中的弓形虫。结果:86例中47例弓形虫阳性,占54.65%。三所医院弓形虫阳性率经统计学处理无显著性差异(P>0.05)。结论:在病理诊断为慢性非特异性淋巴结炎病例中,存在被误诊的弓形虫淋巴结炎病例。
Objective： To research the indirect in situ PCR technology on the diagnosis of toxoplasmosis lymphadenitis. Methods： To design the toxoplasma gondii B1 gene primer, carry on the in situ PCR, hybridization with B1 gene digoxin probe after PCR, test the existence of toxoplasma gondii in the 86 cases which was diagnosed as chronic non-specificity lymphnoditis on pathologic standards. Results： In 86 cases of chronic non-specific lymphnoditis, used in situ PCR technology, 47 cases（54.65% ） showed positive resuits, and there was no significant differences in the three groups （P 〉 0.05 ）. Conclusion： Toxoplasmosis lymphadenitis can be misdiagnosed as chronic non-specific lymphtitis if use the traditional pathologic method.
出处
《江苏大学学报（医学版）》
CAS
2008年第1期70-72,I0002,共4页
Journal of Jiangsu University：Medicine Edition
关键词
弓形虫
淋巴结炎
间接原位PCR
toxoplasma gondii
lymphadenitis
indirect in situ PCR
