摘要
目的 建立全血中环孢素A药物浓度的HPLC测定方法。方法 以Nucleodur C18（150mm×4．6mm，5μm）为分析柱，乙腈-水（71：29，v／v）为流动相，柱温为65℃，检测波长为210nm。先以80％乙腈沉淀蛋白，沉淀后的上清液过SPE小柱处理后进样分析。结果 环孢素A在40．0～1600.0 ng·mL^-1线性关系良好（r＝0．9998），在3个浓度下的平均回收率〉95．0％，日内、日间RSD＜10．0％，环孢素A平均萃取回收率〉86.0％，环孢素D平均萃取回收率为91．2％。结论本方法稳定、准确，能用于测定环孢素A血药浓度。
Objective To establish an HPLC method for assay of cyclosporine A in whole blood. Methods The chromatographic column was Nucleodur C8 （150 mm× 4.6 mm, 5 μm）, the mobile phase consisted of acetonitrile and water （71 ： 29, v/v）, the column temperature was controlled at 65 ℃ and the UV detector was operated at 210 nm. The whole boold samples were added the 80% acetonitrile precipitate albumen, after the SPE subminiature column was disposed , the sample was directly injected into the system. Results The calibration curve was linear in the range of 40. 0～1 600.0 ng· mL^-1. The average recoveries of the three concentrations were all higher than 95.0%. The intra-day RSD and inter-day RSD was both less than 10.0%. the extraction recovery of cyclosporine A was higher than 86. 0%. Conclusion The method is steady and accurate. It is suitable for measuring cyclosporine A in whole blood.
出处
《中南药学》
CAS
2008年第1期74-75,共2页
Central South Pharmacy
关键词
SPE-HPLC
环孢素A
血药浓度
SPE-HPLC Cyclosporine A drug concentration in whole blood
