摘要
目的:基于对Lewis肺癌组织中VEGF-C蛋白及mRNA表达的影响,探讨康莱特注射液抗肿瘤转移的分子机制,为其临床应用提供理论疗效证据。方法:50只C57小鼠接种Lewis肺癌瘤株后,随机分为5组:模型组、CTX组、康莱特低剂量组、康莱特中剂量组、康莱特高剂量组。分别腹腔注射相应药物15d后处死,免疫组化法检测各组小鼠肺癌组织中VEGF-C蛋白的阳性表达,用RT-PCR检测VEGF-CmRNA的表达。结果:模型组、CTX组、康莱特低剂量组、康莱特中剂量组、康莱特高剂量组各组中VEGF-C蛋白的阳性表达率分别为:(9.02±0.63)、(3.85±0.27)、(5.23±0.19)、(3.22±0.08)、(2.02±0.06),各组VEGF-CmRNA/GAPDH比值分别为(2.42±0.08)、(2.17±0.06)、(1.75±0.02)、(0.67±0.05)、(0.46±0.03)。结论:抑制VEGF-C蛋白及mRNA的表达可能是康莱特注射液抗肿瘤转移的分子机制之一。
Objective:To study the mol-mechanisms of KLT injection anti-tumor metabasis, according to the effection that the KLT affect on the VEGF-C proteinum and mRNA of Lewis lung cancer tissue. Methods: The 50 C57 mice v-ere randomly divided into 5 groups after copied Lewis lung cancer model to them: the model group, the chemotherapy group, the LD KLT group, the MD KLT group and the HD KLT group, Sacrifice the mice after inject the diff-medicine to abdominal cavity for 15 days, the immunohistochemistry technique used to detect the expression of VEGF-C proteinum of lung cancer tissue, RT-PCR technique to detect the expression of VEGF-C mRNA, Results: the masc-expression rate of VEGF-C in every group is (9.02± 0.63). (3.85± 0.27). (5.23± 0.19), (3.22± 0.08). (2.02± 0.06) corresponding, the proportionality of gray scale of NF-kB/GAPDH in all groups separately is (2,42± 0.08). (2.17± 0,06). (1.75±0.02). (0.67± 0.05).(0.46± 0.03).Conclusion: It' s maybe the one of the mol-mechanisms of KLT to restrain tumor' s metabasis by inhibiting the masc.-expression of VEGF-C and mRNA.
出处
《实用中西医结合临床》
2008年第1期5-6,共2页
Practical Clinical Journal of Integrated Traditional Chinese and Western Medicine
基金
浙江省中医药管理局基金资助课题(No.2004c065)