摘要
目的:利用液-质联用技术定性分析内蒙古赤芍药材中的化学成分。方法:以甲醇回流提取内蒙古赤芍药材。高效液相色谱条件:色谱柱为 Zorbax sB-C^(18)柱(4.6 mm×250 mm,5 μm),流动相0.2%的醋酸水溶液(A)-0.2%醋酸乙腈溶液(B),梯度洗脱,流速0.5 mL·min^(-1),柱温30℃,二极管阵列检测器(DAD),检测波长230 nm。质谱条件:负离子模式扫描,扫描范围100～2000 amu;氮气流速10 L·min^(-1),雾化温度350 ℃,毛细管电压3500 V,裂解电压100 V。结果:通过高效液相色谱将赤芍化学成分较好地分离,根据紫外光谱可大致判断其化合物类型,由电喷雾质谱得到各成分的相对分子质量,进而推测出其中13个主要成分的可能结构。结论:液-质联用技术能快速定性研究赤芍药材中的化学成分,为赤芍指纹图谱的优化和质量标准的制定提供依据。
Objective ： To analyze the chemical constituents of Paeonia lactiflora Pall. by high performance liquid chromatography - diode array detector electrospray ionization - mass spectrometry （ HPLC - DAD/ESI - MS）. Method：The samples were extracted under reflux with methanol. The chromatographic separation was performed on a Zorbax SB- C18 column （4. 6 mm × 250 mm,5 μm）with a linear gradient elution of acetic acid -water （0.2： 100,v/v） and acetic acid - acetonitrile （0.2： 100,v/v）. The flow rate was 0. 5 mL · min^-1 and column temperature was set at 30℃. The UV detection wavelength was set at 230 nm. The negative ionization mode, tem- perature of the capillary,350 ℃ ,capillary vohage,3500 V; gas （ N2 ） flow, 10 L · min^-1, fragment voltage, 100 V. Relative molecular mass data acquisition was performed from m/z 100 to 2000 in full MS scan mode. Result： The chemical constituents of Paeonia lactiflora Pall. were well isolated by HPLC, thirteen of the major chemical constituents were identified by the ESI - MS and ultraviolet （UV） spectra. Conclusion ： HPLC - DAD/ESI - MS technology can be applied to study the chemical constituents, optimized the characteristic fingerprint and quality control method for this herb.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2008年第2期256-259,共4页
Chinese Journal of Pharmaceutical Analysis
关键词
赤芍
化学成分
液-质联用
Paeonia lactiflora Pall.
chemical constituents
HPLC - DAD/ESI - MS
