摘要
目的:探讨骨髓间充质干细胞对脐血CD34+细胞诱导分化为巨核细胞的影响。方法:骨髓间充质干细胞培养采用低糖型DMEM培养基,待细胞满度达到约80%后加入脐血CD34+细胞在一定的培养体系中进行实验,同时以无骨髓间充质干细胞的相应培养体系作为对照,培养14 d后观察结果。实验中共观察了两种不同的培养体系:基础培养液、基础培养液+白细胞介素-11(IL-11)。其中基础培养液为含血小板生成素(TPO)、白细胞介素-3(IL-3)、干细胞因子(SCF)的低糖型DMEM。培养后单个核细胞数采用细胞计数仪分析,CD41+细胞和血小板检测采用流式细胞仪,血小板功能评价采用凝血酶诱导的血小板凝集实验。结果:与相应的对照组比较,骨髓间充质干细胞实验组单个核细胞数增加不明显(P>0.05),而CD41+细胞和血小板数量有明显的增加(P<0.05)。显微镜下和流式细胞仪上均可观察到凝血酶诱导的血小板凝集现象。结论:骨髓间充质干细胞在实验培养体系中可以促进脐血中CD34+细胞诱导分化为巨核细胞。
Aim： In order to investigate the effect of mesenchymal stem/progenitor cells on proliferation and differentiation towards megakaryocytes of CD34＋ cells from human umbilical cord blood in vitro. Methods： After mesenchymal stem/progenitor cells were advancely planted in DMEM medium and grown up to 80%, then the CD34＋ cells were added to culture with mesenchymal stem/ progenitor cells or without mesenchymal stem/progenitor cells in DMEM for 14 days with TPO ＋ IL-3 ＋ SCF, TPO ＋ IL-3 ＋ SCF ＋ IL-11 respectively. After cultured for 14 days, mononuclear cells （MNCs） were counted by automatic cell analyzer. The number of CD41 ＋ cells and platelets were detected by flow cytometry. Platelets function were assessed through platelet aggregation test which was induced by thrombin. Results： As compared with the control group, the number of MNCs of co-culture system was not increased significantly（ P 〉 0.05）, but the number of CD41 ＋ cells and platelets were increased significantly （P 〈 0.05）. The platelets were aggregated by thrombin induced which could be seen in microscope or flow cytometry. Conclusion： It is concluded that mesenchymal stem/progenitor cells may be promoted to induce the coM blood CD34 ＋ cells to differentiate towards megakaryocyte in the culture medium.
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2008年第1期77-80,共4页
Chinese Journal of Applied Physiology
基金
浙江省科技厅重点项目(2006C23032)
浙江省医药卫生优秀青年科技人才专项基金资助项目(2002QN005)
